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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP429320STN
CSB-EP429320STN
CSB-BP429320STN
CSB-MP429320STN
CSB-EP429320STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosome maturation factor RimP (rimP) CSB-YP429321STN
CSB-EP429321STN
CSB-BP429321STN
CSB-MP429321STN
CSB-EP429321STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Peptidyl-tRNA hydrolase (pth) CSB-YP429322STN
CSB-EP429322STN
CSB-BP429322STN
CSB-MP429322STN
CSB-EP429322STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Octanoyltransferase (lipB) CSB-YP429323STN
CSB-EP429323STN
CSB-BP429323STN
CSB-MP429323STN
CSB-EP429323STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Electron transport complex protein RnfA (rnfA), partial CSB-YP429324STN1
CSB-EP429324STN1
CSB-BP429324STN1
CSB-MP429324STN1
CSB-EP429324STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Na (+)-translocating NADH-quinone reductase subunit D, partial CSB-YP429325STN1
CSB-EP429325STN1
CSB-BP429325STN1
CSB-MP429325STN1
CSB-EP429325STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S9 (rpsI) CSB-YP429326STN
CSB-EP429326STN
CSB-BP429326STN
CSB-MP429326STN
CSB-EP429326STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP429327STN
CSB-EP429327STN
CSB-BP429327STN
CSB-MP429327STN
CSB-EP429327STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP429328STN
CSB-EP429328STN
CSB-BP429328STN
CSB-MP429328STN
CSB-EP429328STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Pantothenate synthetase (panC) CSB-YP429329STN
CSB-EP429329STN
CSB-BP429329STN
CSB-MP429329STN
CSB-EP429329STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cell volume regulation protein A homolog (cvrA), partial CSB-YP429330STN
CSB-EP429330STN
CSB-BP429330STN
CSB-MP429330STN
CSB-EP429330STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Integration host factor subunit beta (ihfB) CSB-YP429331STN
CSB-EP429331STN
CSB-BP429331STN
CSB-MP429331STN
CSB-EP429331STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Adenylyl-sulfate kinase (cysC) CSB-YP429332STN
CSB-EP429332STN
CSB-BP429332STN
CSB-MP429332STN
CSB-EP429332STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP429333STN
CSB-EP429333STN
CSB-BP429333STN
CSB-MP429333STN
CSB-EP429333STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Co-chaperone protein hscB homolog (hscB) CSB-YP429334STN
CSB-EP429334STN
CSB-BP429334STN
CSB-MP429334STN
CSB-EP429334STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L21 (rplU) CSB-YP429335STN
CSB-EP429335STN
CSB-BP429335STN
CSB-MP429335STN
CSB-EP429335STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP phosphoribosyltransferase (hisG) CSB-YP429336STN
CSB-EP429336STN
CSB-BP429336STN
CSB-MP429336STN
CSB-EP429336STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP429337STN
CSB-EP429337STN
CSB-BP429337STN
CSB-MP429337STN
CSB-EP429337STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP429338STN
CSB-EP429338STN
CSB-BP429338STN
CSB-MP429338STN
CSB-EP429338STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0267 protein Sbal195_2637 (Sbal195_2637) CSB-YP429339STN
CSB-EP429339STN
CSB-BP429339STN
CSB-MP429339STN
CSB-EP429339STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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