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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Serine protease splF (splF) CSB-YP428720SUM
CSB-EP428720SUM
CSB-BP428720SUM
CSB-MP428720SUM
CSB-EP428720SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splB (splB) CSB-YP428721SUM
CSB-EP428721SUM
CSB-BP428721SUM
CSB-MP428721SUM
CSB-EP428721SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ketol-acid reductoisomerase (ilvC) CSB-YP428722SUM
CSB-EP428722SUM
CSB-BP428722SUM
CSB-MP428722SUM
CSB-EP428722SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein sprT-like (USA300HOU_2057) CSB-YP428723SUM
CSB-EP428723SUM
CSB-BP428723SUM
CSB-MP428723SUM
CSB-EP428723SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP428724SUM1
CSB-EP428724SUM1
CSB-BP428724SUM1
CSB-MP428724SUM1
CSB-EP428724SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP428725SUM
CSB-EP428725SUM
CSB-BP428725SUM
CSB-MP428725SUM
CSB-EP428725SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazolonepropionase (hutI) CSB-YP428726SUM
CSB-EP428726SUM
CSB-BP428726SUM
CSB-MP428726SUM
CSB-EP428726SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative 3-methyladenine DNA glycosylase (USA300HOU_2325) CSB-YP428727SUM
CSB-EP428727SUM
CSB-BP428727SUM
CSB-MP428727SUM
CSB-EP428727SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nitrate transporter narT (narT), partial CSB-YP428728SUM
CSB-EP428728SUM
CSB-BP428728SUM
CSB-MP428728SUM
CSB-EP428728SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator ArcR (arcR) CSB-YP428729SUM
CSB-EP428729SUM
CSB-BP428729SUM
CSB-MP428729SUM
CSB-EP428729SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP phosphoribosyltransferase (hisG) CSB-YP428730SUM
CSB-EP428730SUM
CSB-BP428730SUM
CSB-MP428730SUM
CSB-EP428730SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0176 protein USA300HOU_2691 (USA300HOU_2691) CSB-YP428731SUM
CSB-EP428731SUM
CSB-BP428731SUM
CSB-MP428731SUM
CSB-EP428731SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP428732SUX
CSB-EP428732SUX
CSB-BP428732SUX
CSB-MP428732SUX
CSB-EP428732SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 50S ribosomal protein L11 (rplK) CSB-YP428733SUX
CSB-EP428733SUX
CSB-BP428733SUX
CSB-MP428733SUX
CSB-EP428733SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 50S ribosomal protein L20 (rplT) CSB-YP428734SUX
CSB-EP428734SUX
CSB-BP428734SUX
CSB-MP428734SUX
CSB-EP428734SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP428735SUX
CSB-EP428735SUX
CSB-BP428735SUX
CSB-MP428735SUX
CSB-EP428735SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri Lon protease (lon), partial CSB-YP428736SUX
CSB-EP428736SUX
CSB-BP428736SUX
CSB-MP428736SUX
CSB-EP428736SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 30S ribosomal protein S21 (rpsU) CSB-YP428737SUX
CSB-EP428737SUX
CSB-BP428737SUX
CSB-MP428737SUX
CSB-EP428737SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP428738SUX
CSB-EP428738SUX
CSB-BP428738SUX
CSB-MP428738SUX
CSB-EP428738SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP428739SUX
CSB-EP428739SUX
CSB-BP428739SUX
CSB-MP428739SUX
CSB-EP428739SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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