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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ceratophyllum demersum Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP427960CWK1
CSB-EP427960CWK1
CSB-BP427960CWK1
CSB-MP427960CWK1
CSB-EP427960CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP427961CWK1
CSB-EP427961CWK1
CSB-BP427961CWK1
CSB-MP427961CWK1
CSB-EP427961CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata ATP synthase subunit alpha, plastid (atpA), partial CSB-YP427962CXQ
CSB-EP427962CXQ
CSB-BP427962CXQ
CSB-MP427962CXQ
CSB-EP427962CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP427963CXQ
CSB-EP427963CXQ
CSB-BP427963CXQ
CSB-MP427963CXQ
CSB-EP427963CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Apocytochrome f (petA), partial CSB-YP427964CXQ1
CSB-EP427964CXQ1
CSB-BP427964CXQ1
CSB-MP427964CXQ1
CSB-EP427964CXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata ATP synthase subunit beta, plastid (atpB) CSB-YP427965CXQ
CSB-EP427965CXQ
CSB-BP427965CXQ
CSB-MP427965CXQ
CSB-EP427965CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Plastid 30S ribosomal protein S11 (rps11) CSB-YP427966CXQ
CSB-EP427966CXQ
CSB-BP427966CXQ
CSB-MP427966CXQ
CSB-EP427966CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Plastid 30S ribosomal protein S3 (rps3) CSB-YP427967CXQ
CSB-EP427967CXQ
CSB-BP427967CXQ
CSB-MP427967CXQ
CSB-EP427967CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata 30S ribosomal protein S7, plastid (rps7-A) CSB-YP427968CXQ
CSB-EP427968CXQ
CSB-BP427968CXQ
CSB-MP427968CXQ
CSB-EP427968CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora ATP synthase subunit alpha, plastid (atpA), partial CSB-YP427969CXS
CSB-EP427969CXS
CSB-BP427969CXS
CSB-MP427969CXS
CSB-EP427969CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP427970CXS1
CSB-EP427970CXS1
CSB-BP427970CXS1
CSB-MP427970CXS1
CSB-EP427970CXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP427971CXS1
CSB-EP427971CXS1
CSB-BP427971CXS1
CSB-MP427971CXS1
CSB-EP427971CXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora 50S ribosomal protein L33, plastid (rpl33) CSB-YP427972CXS
CSB-EP427972CXS
CSB-BP427972CXS
CSB-MP427972CXS
CSB-EP427972CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Plastid 30S ribosomal protein S11 (rps11) CSB-YP427973CXS
CSB-EP427973CXS
CSB-BP427973CXS
CSB-MP427973CXS
CSB-EP427973CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora 30S ribosomal protein S7, plastid (rps7-A) CSB-YP427974CXS
CSB-EP427974CXS
CSB-BP427974CXS
CSB-MP427974CXS
CSB-EP427974CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cholesterol 25-hydroxylase-like protein 2 (ch25hl2), partial CSB-YP427975DIL1
CSB-EP427975DIL1
CSB-BP427975DIL1
CSB-MP427975DIL1
CSB-EP427975DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CID domain-containing protein 1 (cids-1) CSB-YP427976CXX
CSB-EP427976CXX
CSB-BP427976CXX
CSB-MP427976CXX
CSB-EP427976CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein smg-8 (smg-8), partial CSB-YP427977CXX
CSB-EP427977CXX
CSB-BP427977CXX
CSB-MP427977CXX
CSB-EP427977CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative exosome complex component RRP41 (exos-4.1) CSB-YP427978CXX
CSB-EP427978CXX
CSB-BP427978CXX
CSB-MP427978CXX
CSB-EP427978CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative cuticle collagen 99 (col-99), partial CSB-YP427979CXX
CSB-EP427979CXX
CSB-BP427979CXX
CSB-MP427979CXX
CSB-EP427979CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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