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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ceratophyllum demersum Photosystem II D2 protein (psbD), partial CSB-YP428160CWK1
CSB-EP428160CWK1
CSB-BP428160CWK1
CSB-MP428160CWK1
CSB-EP428160CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP428161CWK
CSB-EP428161CWK
CSB-BP428161CWK
CSB-MP428161CWK
CSB-EP428161CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Chloroplast envelope membrane protein (cemA), partial CSB-YP428162CWK1
CSB-EP428162CWK1
CSB-BP428162CWK1
CSB-MP428162CWK1
CSB-EP428162CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP428163CWK
CSB-EP428163CWK
CSB-BP428163CWK
CSB-MP428163CWK
CSB-EP428163CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP428164CWK
CSB-EP428164CWK
CSB-BP428164CWK
CSB-MP428164CWK
CSB-EP428164CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum NAD (P)H-quinone oxidoreductase chain 4, chloroplastic, partial CSB-YP428165CWK
CSB-EP428165CWK
CSB-BP428165CWK
CSB-MP428165CWK
CSB-EP428165CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hirudo medicinalis Neuromacin CSB-YP428166HSM
CSB-EP428166HSM
CSB-BP428166HSM
CSB-MP428166HSM
CSB-EP428166HSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP428167CXQ1
CSB-EP428167CXQ1
CSB-BP428167CXQ1
CSB-MP428167CXQ1
CSB-EP428167CXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Photosystem I assembly protein ycf3 (ycf3) CSB-YP428168CXQ
CSB-EP428168CXQ
CSB-BP428168CXQ
CSB-MP428168CXQ
CSB-EP428168CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP428169CXQ1
CSB-EP428169CXQ1
CSB-BP428169CXQ1
CSB-MP428169CXQ1
CSB-EP428169CXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Plastid 30S ribosomal protein S18 (rps18) CSB-YP428170CXQ
CSB-EP428170CXQ
CSB-BP428170CXQ
CSB-MP428170CXQ
CSB-EP428170CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP428171CXQ
CSB-EP428171CXQ
CSB-BP428171CXQ
CSB-MP428171CXQ
CSB-EP428171CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata 30S ribosomal protein S19, plastid (rps19) CSB-YP428172CXQ
CSB-EP428172CXQ
CSB-BP428172CXQ
CSB-MP428172CXQ
CSB-EP428172CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta exaltata Putative membrane protein ycf1 (ycf1), partial CSB-YP428173CXQ
CSB-EP428173CXQ
CSB-BP428173CXQ
CSB-MP428173CXQ
CSB-EP428173CXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Photosystem II reaction center protein M (psbM), partial CSB-YP428174CXS
CSB-EP428174CXS
CSB-BP428174CXS
CSB-MP428174CXS
CSB-EP428174CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP428175CXS
CSB-EP428175CXS
CSB-BP428175CXS
CSB-MP428175CXS
CSB-EP428175CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Photosystem II reaction center protein J (psbJ), partial CSB-YP428176CXS
CSB-EP428176CXS
CSB-BP428176CXS
CSB-MP428176CXS
CSB-EP428176CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora 50S ribosomal protein L20, plastid (rpl20) CSB-YP428177CXS
CSB-EP428177CXS
CSB-BP428177CXS
CSB-MP428177CXS
CSB-EP428177CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Plastid 30S ribosomal protein S3 (rps3) CSB-YP428178CXS
CSB-EP428178CXS
CSB-BP428178CXS
CSB-MP428178CXS
CSB-EP428178CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Putative membrane protein ycf1 (ycf1), partial CSB-YP428179CXS
CSB-EP428179CXS
CSB-BP428179CXS
CSB-MP428179CXS
CSB-EP428179CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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