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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gluconacetobacter diazotrophicus Enolase (eno) CSB-YP429160GEN
CSB-EP429160GEN
CSB-BP429160GEN
CSB-MP429160GEN
CSB-EP429160GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP429161GEN
CSB-EP429161GEN
CSB-BP429161GEN
CSB-MP429161GEN
CSB-EP429161GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP429162GEN1
CSB-EP429162GEN1
CSB-BP429162GEN1
CSB-MP429162GEN1
CSB-EP429162GEN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP429163GEN
CSB-EP429163GEN
CSB-BP429163GEN
CSB-MP429163GEN
CSB-EP429163GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus tRNA (guanine-N (1)-)-methyltransferase CSB-YP429164GEN
CSB-EP429164GEN
CSB-BP429164GEN
CSB-MP429164GEN
CSB-EP429164GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Arginine--tRNA ligase (argS), partial CSB-YP429165BPB
CSB-EP429165BPB
CSB-BP429165BPB
CSB-MP429165BPB
CSB-EP429165BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP429166BPB
CSB-EP429166BPB
CSB-BP429166BPB
CSB-MP429166BPB
CSB-EP429166BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Undecaprenyl-diphosphatase (uppP), partial CSB-YP429167BPB1
CSB-EP429167BPB1
CSB-BP429167BPB1
CSB-MP429167BPB1
CSB-EP429167BPB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii ATP synthase gamma chain (atpG) CSB-YP429168BPB
CSB-EP429168BPB
CSB-BP429168BPB
CSB-MP429168BPB
CSB-EP429168BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0133 protein Bpet3552 (Bpet3552) CSB-YP429169BPB
CSB-EP429169BPB
CSB-BP429169BPB
CSB-MP429169BPB
CSB-EP429169BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Probable rRNA maturation factor (Bpet3656) CSB-YP429170BPB
CSB-EP429170BPB
CSB-BP429170BPB
CSB-MP429170BPB
CSB-EP429170BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Glutamyl-tRNA (Gln) amidotransferase subunit A, partial CSB-YP429171BPB
CSB-EP429171BPB
CSB-BP429171BPB
CSB-MP429171BPB
CSB-EP429171BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0301 protein Bpet0561 (Bpet0561) CSB-YP429172BPB
CSB-EP429172BPB
CSB-BP429172BPB
CSB-MP429172BPB
CSB-EP429172BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L13 (rplM) CSB-YP429173BPB
CSB-EP429173BPB
CSB-BP429173BPB
CSB-MP429173BPB
CSB-EP429173BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Transcriptional repressor NrdR (nrdR) CSB-YP429174BPB
CSB-EP429174BPB
CSB-BP429174BPB
CSB-MP429174BPB
CSB-EP429174BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii D-alanine--D-alanine ligase (ddl) CSB-YP429175BPB
CSB-EP429175BPB
CSB-BP429175BPB
CSB-MP429175BPB
CSB-EP429175BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP429176BPB
CSB-EP429176BPB
CSB-BP429176BPB
CSB-MP429176BPB
CSB-EP429176BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Probable septum site-determining protein MinC (minC) CSB-YP429177BPB
CSB-EP429177BPB
CSB-BP429177BPB
CSB-MP429177BPB
CSB-EP429177BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP429178BPB
CSB-EP429178BPB
CSB-BP429178BPB
CSB-MP429178BPB
CSB-EP429178BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Carboxylate-amine ligase Bpet4705 (Bpet4705) CSB-YP429179BPB
CSB-EP429179BPB
CSB-BP429179BPB
CSB-MP429179BPB
CSB-EP429179BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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