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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP431983STN
CSB-EP431983STN
CSB-BP431983STN
CSB-MP431983STN
CSB-EP431983STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0345 protein Sbal195_0273 (Sbal195_0273) CSB-YP431984STN
CSB-EP431984STN
CSB-BP431984STN
CSB-MP431984STN
CSB-EP431984STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP431985STN
CSB-EP431985STN
CSB-BP431985STN
CSB-MP431985STN
CSB-EP431985STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Histidine--tRNA ligase (hisS) CSB-YP431986STN
CSB-EP431986STN
CSB-BP431986STN
CSB-MP431986STN
CSB-EP431986STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribonuclease PH (rph) CSB-YP431987STN
CSB-EP431987STN
CSB-BP431987STN
CSB-MP431987STN
CSB-EP431987STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP431988STN
CSB-EP431988STN
CSB-BP431988STN
CSB-MP431988STN
CSB-EP431988STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP431989STN
CSB-EP431989STN
CSB-BP431989STN
CSB-MP431989STN
CSB-EP431989STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0178 protein Sbal195_1808 (Sbal195_1808) CSB-YP431990STN
CSB-EP431990STN
CSB-BP431990STN
CSB-MP431990STN
CSB-EP431990STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Alanine--tRNA ligase (alaS), partial CSB-YP431991STN
CSB-EP431991STN
CSB-BP431991STN
CSB-MP431991STN
CSB-EP431991STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP431992STN
CSB-EP431992STN
CSB-BP431992STN
CSB-MP431992STN
CSB-EP431992STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L31 (rpmE) CSB-YP431993STN
CSB-EP431993STN
CSB-BP431993STN
CSB-MP431993STN
CSB-EP431993STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0745 protein Sbal195_1903 (Sbal195_1903) CSB-YP431994STN
CSB-EP431994STN
CSB-BP431994STN
CSB-MP431994STN
CSB-EP431994STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L25 (rplY) CSB-YP431995STN
CSB-EP431995STN
CSB-BP431995STN
CSB-MP431995STN
CSB-EP431995STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP431996STN
CSB-EP431996STN
CSB-BP431996STN
CSB-MP431996STN
CSB-EP431996STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribose-5-phosphate isomerase A (rpiA) CSB-YP431997STN
CSB-EP431997STN
CSB-BP431997STN
CSB-MP431997STN
CSB-EP431997STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chaperone protein DnaK (dnaK), partial CSB-YP431998STN
CSB-EP431998STN
CSB-BP431998STN
CSB-MP431998STN
CSB-EP431998STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP431999STN
CSB-EP431999STN
CSB-BP431999STN
CSB-MP431999STN
CSB-EP431999STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Elongation factor P (efp) CSB-YP432000STN
CSB-EP432000STN
CSB-BP432000STN
CSB-MP432000STN
CSB-EP432000STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP432001STN
CSB-EP432001STN
CSB-BP432001STN
CSB-MP432001STN
CSB-EP432001STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein CrcB homolog (crcB), partial CSB-YP432002STN1
CSB-EP432002STN1
CSB-BP432002STN1
CSB-MP432002STN1
CSB-EP432002STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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