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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Physcomitrella patens subsp. patens Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (PHYPADRAFT_213576), partial CSB-YP432383EVD
CSB-EP432383EVD
CSB-BP432383EVD
CSB-MP432383EVD
CSB-EP432383EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Adenylosuccinate synthetase 2, chloroplastic (PURA2) CSB-YP432384EVD
CSB-EP432384EVD
CSB-BP432384EVD
CSB-MP432384EVD
CSB-EP432384EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Arabinogalactan peptide 3 (AGPEP3) CSB-YP432385OFG
CSB-EP432385OFG
CSB-BP432385OFG
CSB-MP432385OFG
CSB-EP432385OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Ribosome biogenesis protein WDR12 homolog (30764) CSB-YP432386MOD
CSB-EP432386MOD
CSB-BP432386MOD
CSB-MP432386MOD
CSB-EP432386MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Adenylosuccinate synthetase (20027) CSB-YP432387MOD
CSB-EP432387MOD
CSB-BP432387MOD
CSB-MP432387MOD
CSB-EP432387MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Lissencephaly-1 homolog (35260) CSB-YP432388MOD
CSB-EP432388MOD
CSB-BP432388MOD
CSB-MP432388MOD
CSB-EP432388MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP432389BON
CSB-EP432389BON
CSB-BP432389BON
CSB-MP432389BON
CSB-EP432389BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Biotin synthase (bioB) CSB-YP432390BON
CSB-EP432390BON
CSB-BP432390BON
CSB-MP432390BON
CSB-EP432390BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP432391BON
CSB-EP432391BON
CSB-BP432391BON
CSB-MP432391BON
CSB-EP432391BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 3-dehydroquinate dehydratase (aroQ) CSB-YP432392BON
CSB-EP432392BON
CSB-BP432392BON
CSB-MP432392BON
CSB-EP432392BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S21 (rpsU) CSB-YP432393BON
CSB-EP432393BON
CSB-BP432393BON
CSB-MP432393BON
CSB-EP432393BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP432394BON
CSB-EP432394BON
CSB-BP432394BON
CSB-MP432394BON
CSB-EP432394BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Uridine kinase (udk) CSB-YP432395BON
CSB-EP432395BON
CSB-BP432395BON
CSB-MP432395BON
CSB-EP432395BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Lactate utilization protein B 1 (lutB1) CSB-YP432396BON
CSB-EP432396BON
CSB-BP432396BON
CSB-MP432396BON
CSB-EP432396BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Quinolinate synthase A (nadA) CSB-YP432397BON
CSB-EP432397BON
CSB-BP432397BON
CSB-MP432397BON
CSB-EP432397BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glutamyl-tRNA reductase (hemA) CSB-YP432398BON
CSB-EP432398BON
CSB-BP432398BON
CSB-MP432398BON
CSB-EP432398BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0234 protein BcerKBAB4_1061 (BcerKBAB4_1061) CSB-YP432399BON
CSB-EP432399BON
CSB-BP432399BON
CSB-MP432399BON
CSB-EP432399BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Small, acid-soluble spore protein I (sspI) CSB-YP432400BON
CSB-EP432400BON
CSB-BP432400BON
CSB-MP432400BON
CSB-EP432400BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Indole-3-glycerol phosphate synthase (trpC) CSB-YP432401BON
CSB-EP432401BON
CSB-BP432401BON
CSB-MP432401BON
CSB-EP432401BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP432402BON
CSB-EP432402BON
CSB-BP432402BON
CSB-MP432402BON
CSB-EP432402BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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