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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sorangium cellulosum ATP-dependent zinc metalloprotease FtsH 1 (ftsH1), partial CSB-YP433583SUD
CSB-EP433583SUD
CSB-BP433583SUD
CSB-MP433583SUD
CSB-EP433583SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum UPF0059 membrane protein sce0548 (sce0548), partial CSB-YP433584SUD1
CSB-EP433584SUD1
CSB-BP433584SUD1
CSB-MP433584SUD1
CSB-EP433584SUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Aspartate carbamoyltransferase (pyrB) CSB-YP433585SUD
CSB-EP433585SUD
CSB-BP433585SUD
CSB-MP433585SUD
CSB-EP433585SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Glutamate--tRNA ligase 1 (gltX1) CSB-YP433586GEN
CSB-EP433586GEN
CSB-BP433586GEN
CSB-MP433586GEN
CSB-EP433586GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP433587GEN
CSB-EP433587GEN
CSB-BP433587GEN
CSB-MP433587GEN
CSB-EP433587GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Na (+)/H (+) antiporter nhaA, partial CSB-YP433588GEN
CSB-EP433588GEN
CSB-BP433588GEN
CSB-MP433588GEN
CSB-EP433588GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L15 (rplO) CSB-YP433589GEN
CSB-EP433589GEN
CSB-BP433589GEN
CSB-MP433589GEN
CSB-EP433589GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L14 (rplN) CSB-YP433590GEN
CSB-EP433590GEN
CSB-BP433590GEN
CSB-MP433590GEN
CSB-EP433590GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L4 (rplD) CSB-YP433591GEN
CSB-EP433591GEN
CSB-BP433591GEN
CSB-MP433591GEN
CSB-EP433591GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L7/L12 (rplL) CSB-YP433592GEN
CSB-EP433592GEN
CSB-BP433592GEN
CSB-MP433592GEN
CSB-EP433592GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus UPF0060 membrane protein GDI3492/Gdia_2889 (GDI3492, Gdia_2889), partial CSB-YP433593GEN1
CSB-EP433593GEN1
CSB-BP433593GEN1
CSB-MP433593GEN1
CSB-EP433593GEN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP433594GEN
CSB-EP433594GEN
CSB-BP433594GEN
CSB-MP433594GEN
CSB-EP433594GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Ribonuclease D (rnd) CSB-YP433595GEN
CSB-EP433595GEN
CSB-BP433595GEN
CSB-MP433595GEN
CSB-EP433595GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Phosphoglucosamine mutase (glmM) CSB-YP433596GEN
CSB-EP433596GEN
CSB-BP433596GEN
CSB-MP433596GEN
CSB-EP433596GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus PKHD-type hydroxylase GDI1238/Gdia_1949 (GDI1238, Gdia_1949) CSB-YP433597GEN
CSB-EP433597GEN
CSB-BP433597GEN
CSB-MP433597GEN
CSB-EP433597GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Coenzyme PQQ synthesis protein A (pqqA) CSB-YP433598GEN
CSB-EP433598GEN
CSB-BP433598GEN
CSB-MP433598GEN
CSB-EP433598GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S15 (rpsO) CSB-YP433599GEN
CSB-EP433599GEN
CSB-BP433599GEN
CSB-MP433599GEN
CSB-EP433599GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L31 (rpmE) CSB-YP433600GEN
CSB-EP433600GEN
CSB-BP433600GEN
CSB-MP433600GEN
CSB-EP433600GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP433601GEN
CSB-EP433601GEN
CSB-BP433601GEN
CSB-MP433601GEN
CSB-EP433601GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus UPF0102 protein GDI1964/Gdia_0189 (GDI1964, Gdia_0189) CSB-YP433602GEN
CSB-EP433602GEN
CSB-BP433602GEN
CSB-MP433602GEN
CSB-EP433602GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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