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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella canis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP432083BPH
CSB-EP432083BPH
CSB-BP432083BPH
CSB-MP432083BPH
CSB-EP432083BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 30S ribosomal protein S18 (rpsR) CSB-YP432084BPH
CSB-EP432084BPH
CSB-BP432084BPH
CSB-MP432084BPH
CSB-EP432084BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP432085BPH
CSB-EP432085BPH
CSB-BP432085BPH
CSB-MP432085BPH
CSB-EP432085BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Betaine aldehyde dehydrogenase (betB) CSB-YP432086BPH
CSB-EP432086BPH
CSB-BP432086BPH
CSB-MP432086BPH
CSB-EP432086BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP432087BPH
CSB-EP432087BPH
CSB-BP432087BPH
CSB-MP432087BPH
CSB-EP432087BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Probable rRNA maturation factor (BCAN_A2198) CSB-YP432088BPH
CSB-EP432088BPH
CSB-BP432088BPH
CSB-MP432088BPH
CSB-EP432088BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Porin omp2b (omp2b) CSB-YP432089BPH
CSB-EP432089BPH
CSB-BP432089BPH
CSB-MP432089BPH
CSB-EP432089BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Heme A synthase (ctaA), partial CSB-YP432090BPH
CSB-EP432090BPH
CSB-BP432090BPH
CSB-MP432090BPH
CSB-EP432090BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP432091BPH
CSB-EP432091BPH
CSB-BP432091BPH
CSB-MP432091BPH
CSB-EP432091BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Arginine--tRNA ligase (argS), partial CSB-YP432092BPH
CSB-EP432092BPH
CSB-BP432092BPH
CSB-MP432092BPH
CSB-EP432092BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 3-dehydroquinate dehydratase (aroQ) CSB-YP432093BPH
CSB-EP432093BPH
CSB-BP432093BPH
CSB-MP432093BPH
CSB-EP432093BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP432094BPH1
CSB-EP432094BPH1
CSB-BP432094BPH1
CSB-MP432094BPH1
CSB-EP432094BPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Uronate isomerase (uxaC) CSB-YP432095BPH
CSB-EP432095BPH
CSB-BP432095BPH
CSB-MP432095BPH
CSB-EP432095BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP432096BPH1
CSB-EP432096BPH1
CSB-BP432096BPH1
CSB-MP432096BPH1
CSB-EP432096BPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Uracil phosphoribosyltransferase (upp) CSB-YP432097BPH
CSB-EP432097BPH
CSB-BP432097BPH
CSB-MP432097BPH
CSB-EP432097BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Histidine--tRNA ligase (hisS), partial CSB-YP432098BPH
CSB-EP432098BPH
CSB-BP432098BPH
CSB-MP432098BPH
CSB-EP432098BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Cell division topological specificity factor (minE) CSB-YP432099BPH
CSB-EP432099BPH
CSB-BP432099BPH
CSB-MP432099BPH
CSB-EP432099BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Multidrug resistance protein MdtK (mdtK), partial CSB-YP432100STE
CSB-EP432100STE
CSB-BP432100STE
CSB-MP432100STE
CSB-EP432100STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP432101STE
CSB-EP432101STE
CSB-BP432101STE
CSB-MP432101STE
CSB-EP432101STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP432102STE
CSB-EP432102STE
CSB-BP432102STE
CSB-MP432102STE
CSB-EP432102STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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