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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Renibacterium salmoninarum Chaperone protein DnaK (dnaK), partial CSB-YP432483RIH
CSB-EP432483RIH
CSB-BP432483RIH
CSB-MP432483RIH
CSB-EP432483RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum DNA replication and repair protein recF (recF) CSB-YP432484RIH
CSB-EP432484RIH
CSB-BP432484RIH
CSB-MP432484RIH
CSB-EP432484RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Cell division protein SepF (sepF) CSB-YP432485RIH
CSB-EP432485RIH
CSB-BP432485RIH
CSB-MP432485RIH
CSB-EP432485RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP432486RIH
CSB-EP432486RIH
CSB-BP432486RIH
CSB-MP432486RIH
CSB-EP432486RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP432487RIH
CSB-EP432487RIH
CSB-BP432487RIH
CSB-MP432487RIH
CSB-EP432487RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Proteasome subunit beta (prcB) CSB-YP432488RIH
CSB-EP432488RIH
CSB-BP432488RIH
CSB-MP432488RIH
CSB-EP432488RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP432489RIH
CSB-EP432489RIH
CSB-BP432489RIH
CSB-MP432489RIH
CSB-EP432489RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 50S ribosomal protein L36 2 (rpmJ2) CSB-YP432490RIH
CSB-EP432490RIH
CSB-BP432490RIH
CSB-MP432490RIH
CSB-EP432490RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 30S ribosomal protein S8 (rpsH) CSB-YP432491RIH
CSB-EP432491RIH
CSB-BP432491RIH
CSB-MP432491RIH
CSB-EP432491RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 30S ribosomal protein S3 (rpsC) CSB-YP432492RIH
CSB-EP432492RIH
CSB-BP432492RIH
CSB-MP432492RIH
CSB-EP432492RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP432493RIH
CSB-EP432493RIH
CSB-BP432493RIH
CSB-MP432493RIH
CSB-EP432493RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum UvrABC system protein C (uvrC), partial CSB-YP432494RIH
CSB-EP432494RIH
CSB-BP432494RIH
CSB-MP432494RIH
CSB-EP432494RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 30S ribosomal protein S18 (rpsR) CSB-YP432495RIH
CSB-EP432495RIH
CSB-BP432495RIH
CSB-MP432495RIH
CSB-EP432495RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis D-amino acid dehydrogenase small subunit (dadA) CSB-YP432496BWU
CSB-EP432496BWU
CSB-BP432496BWU
CSB-MP432496BWU
CSB-EP432496BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 50S ribosomal protein L34 (rpmH) CSB-YP432497BWU
CSB-EP432497BWU
CSB-BP432497BWU
CSB-MP432497BWU
CSB-EP432497BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Protein TolB (tolB) CSB-YP432498BWU
CSB-EP432498BWU
CSB-BP432498BWU
CSB-MP432498BWU
CSB-EP432498BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 4-hydroxyproline epimerase (BSUIS_B1268) CSB-YP432499BWU
CSB-EP432499BWU
CSB-BP432499BWU
CSB-MP432499BWU
CSB-EP432499BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Gamma-glutamyl phosphate reductase (proA) CSB-YP432500BWU
CSB-EP432500BWU
CSB-BP432500BWU
CSB-MP432500BWU
CSB-EP432500BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 50S ribosomal protein L27 (rpmA) CSB-YP432501BWU
CSB-EP432501BWU
CSB-BP432501BWU
CSB-MP432501BWU
CSB-EP432501BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP432502BWU
CSB-EP432502BWU
CSB-BP432502BWU
CSB-MP432502BWU
CSB-EP432502BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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