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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae ATP-dependent RNA helicase rhlB (rhlB) CSB-YP432123STE
CSB-EP432123STE
CSB-BP432123STE
CSB-MP432123STE
CSB-EP432123STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP432124STE1
CSB-EP432124STE1
CSB-BP432124STE1
CSB-MP432124STE1
CSB-EP432124STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP432125STE
CSB-EP432125STE
CSB-BP432125STE
CSB-MP432125STE
CSB-EP432125STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Imidazolonepropionase (hutI) CSB-YP432126STE
CSB-EP432126STE
CSB-BP432126STE
CSB-MP432126STE
CSB-EP432126STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Low affinity potassium transport system protein kup (kup), partial CSB-YP432127STE
CSB-EP432127STE
CSB-BP432127STE
CSB-MP432127STE
CSB-EP432127STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae ATP synthase gamma chain (atpG) CSB-YP432128STE
CSB-EP432128STE
CSB-BP432128STE
CSB-MP432128STE
CSB-EP432128STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L34 (rpmH) CSB-YP432129STE
CSB-EP432129STE
CSB-BP432129STE
CSB-MP432129STE
CSB-EP432129STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP432130STE
CSB-EP432130STE
CSB-BP432130STE
CSB-MP432130STE
CSB-EP432130STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Probable endonuclease 4 (nfo) CSB-YP432131STE
CSB-EP432131STE
CSB-BP432131STE
CSB-MP432131STE
CSB-EP432131STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP432132STE
CSB-EP432132STE
CSB-BP432132STE
CSB-MP432132STE
CSB-EP432132STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP432133STE
CSB-EP432133STE
CSB-BP432133STE
CSB-MP432133STE
CSB-EP432133STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0758 protein yicR (yicR) CSB-YP432134STE
CSB-EP432134STE
CSB-BP432134STE
CSB-MP432134STE
CSB-EP432134STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Phosphopantetheine adenylyltransferase (coaD) CSB-YP432135STE
CSB-EP432135STE
CSB-BP432135STE
CSB-MP432135STE
CSB-EP432135STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Deoxycytidine triphosphate deaminase (dcd) CSB-YP432136STE
CSB-EP432136STE
CSB-BP432136STE
CSB-MP432136STE
CSB-EP432136STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cysteine--tRNA ligase (cysS) CSB-YP432137STE
CSB-EP432137STE
CSB-BP432137STE
CSB-MP432137STE
CSB-EP432137STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Phosphate propanoyltransferase (pduL) CSB-YP432138STE
CSB-EP432138STE
CSB-BP432138STE
CSB-MP432138STE
CSB-EP432138STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Protein mtfA (mtfA) CSB-YP432139STE
CSB-EP432139STE
CSB-BP432139STE
CSB-MP432139STE
CSB-EP432139STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 7-cyano-7-deazaguanine synthase (queC) CSB-YP432140STE
CSB-EP432140STE
CSB-BP432140STE
CSB-MP432140STE
CSB-EP432140STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP432141STE
CSB-EP432141STE
CSB-BP432141STE
CSB-MP432141STE
CSB-EP432141STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP432142STE
CSB-EP432142STE
CSB-BP432142STE
CSB-MP432142STE
CSB-EP432142STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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