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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP433503PZC
CSB-EP433503PZC
CSB-BP433503PZC
CSB-MP433503PZC
CSB-EP433503PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA ligase (ligA), partial CSB-YP433504PZC
CSB-EP433504PZC
CSB-BP433504PZC
CSB-MP433504PZC
CSB-EP433504PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP433505PZC
CSB-EP433505PZC
CSB-BP433505PZC
CSB-MP433505PZC
CSB-EP433505PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus GTPase obg (obg) CSB-YP433506PZC
CSB-EP433506PZC
CSB-BP433506PZC
CSB-MP433506PZC
CSB-EP433506PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP433507PZC
CSB-EP433507PZC
CSB-BP433507PZC
CSB-MP433507PZC
CSB-EP433507PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b559 subunit beta (psbF), partial CSB-YP433508PZC
CSB-EP433508PZC
CSB-BP433508PZC
CSB-MP433508PZC
CSB-EP433508PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP433509PZC1
CSB-EP433509PZC1
CSB-BP433509PZC1
CSB-MP433509PZC1
CSB-EP433509PZC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP433510PZC
CSB-EP433510PZC
CSB-BP433510PZC
CSB-MP433510PZC
CSB-EP433510PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate--tRNA ligase (gltX) CSB-YP433511PZC
CSB-EP433511PZC
CSB-BP433511PZC
CSB-MP433511PZC
CSB-EP433511PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP433512PYI
CSB-EP433512PYI
CSB-BP433512PYI
CSB-MP433512PYI
CSB-EP433512PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP433513PYI
CSB-EP433513PYI
CSB-BP433513PYI
CSB-MP433513PYI
CSB-EP433513PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L1 (rplA) CSB-YP433514PYI
CSB-EP433514PYI
CSB-BP433514PYI
CSB-MP433514PYI
CSB-EP433514PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP433515PYI
CSB-EP433515PYI
CSB-BP433515PYI
CSB-MP433515PYI
CSB-EP433515PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L36 (rpmJ) CSB-YP433516PYI
CSB-EP433516PYI
CSB-BP433516PYI
CSB-MP433516PYI
CSB-EP433516PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP433517PYI
CSB-EP433517PYI
CSB-BP433517PYI
CSB-MP433517PYI
CSB-EP433517PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Pmob_1549) CSB-YP433518PYI
CSB-EP433518PYI
CSB-BP433518PYI
CSB-MP433518PYI
CSB-EP433518PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Cysteine--tRNA ligase (cysS) CSB-YP433519PYI
CSB-EP433519PYI
CSB-BP433519PYI
CSB-MP433519PYI
CSB-EP433519PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis L-lactate dehydrogenase (ldh) CSB-YP433520PYI
CSB-EP433520PYI
CSB-BP433520PYI
CSB-MP433520PYI
CSB-EP433520PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L16 (rplP) CSB-YP433521PYI
CSB-EP433521PYI
CSB-BP433521PYI
CSB-MP433521PYI
CSB-EP433521PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP433522PYI
CSB-EP433522PYI
CSB-BP433522PYI
CSB-MP433522PYI
CSB-EP433522PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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