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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sorangium cellulosum Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP435304SUD
CSB-EP435304SUD
CSB-BP435304SUD
CSB-MP435304SUD
CSB-EP435304SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L34 (rpmH) CSB-YP435305SUD
CSB-EP435305SUD
CSB-BP435305SUD
CSB-MP435305SUD
CSB-EP435305SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Ribosome-binding factor A (rbfA) CSB-YP435306SUD
CSB-EP435306SUD
CSB-BP435306SUD
CSB-MP435306SUD
CSB-EP435306SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum ATP-dependent zinc metalloprotease FtsH 3 (ftsH3), partial CSB-YP435307SUD1
CSB-EP435307SUD1
CSB-BP435307SUD1
CSB-MP435307SUD1
CSB-EP435307SUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Protein CrcB homolog (crcB), partial CSB-YP435308SUD1
CSB-EP435308SUD1
CSB-BP435308SUD1
CSB-MP435308SUD1
CSB-EP435308SUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Cell division topological specificity factor (minE) CSB-YP435309SUD
CSB-EP435309SUD
CSB-BP435309SUD
CSB-MP435309SUD
CSB-EP435309SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Acetylglutamate kinase (argB) CSB-YP435310SUD
CSB-EP435310SUD
CSB-BP435310SUD
CSB-MP435310SUD
CSB-EP435310SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP435311SUD
CSB-EP435311SUD
CSB-BP435311SUD
CSB-MP435311SUD
CSB-EP435311SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Glutamate--tRNA ligase (gltX), partial CSB-YP435312SUD
CSB-EP435312SUD
CSB-BP435312SUD
CSB-MP435312SUD
CSB-EP435312SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L25 (rplY) CSB-YP435313SUD
CSB-EP435313SUD
CSB-BP435313SUD
CSB-MP435313SUD
CSB-EP435313SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP435314GEN
CSB-EP435314GEN
CSB-BP435314GEN
CSB-MP435314GEN
CSB-EP435314GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP435315GEN
CSB-EP435315GEN
CSB-BP435315GEN
CSB-MP435315GEN
CSB-EP435315GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L9 (rplI) CSB-YP435316GEN
CSB-EP435316GEN
CSB-BP435316GEN
CSB-MP435316GEN
CSB-EP435316GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus UPF0317 protein GDI0188/Gdia_2258 (GDI0188, Gdia_2258) CSB-YP435317GEN
CSB-EP435317GEN
CSB-BP435317GEN
CSB-MP435317GEN
CSB-EP435317GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S13 (rpsM) CSB-YP435318GEN
CSB-EP435318GEN
CSB-BP435318GEN
CSB-MP435318GEN
CSB-EP435318GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L6 (rplF) CSB-YP435319GEN
CSB-EP435319GEN
CSB-BP435319GEN
CSB-MP435319GEN
CSB-EP435319GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 50S ribosomal protein L22 (rplV) CSB-YP435320GEN
CSB-EP435320GEN
CSB-BP435320GEN
CSB-MP435320GEN
CSB-EP435320GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S7 (rpsG) CSB-YP435321GEN
CSB-EP435321GEN
CSB-BP435321GEN
CSB-MP435321GEN
CSB-EP435321GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus UPF0133 protein GDI3467/Gdia_2910 (GDI3467, Gdia_2910) CSB-YP435322GEN
CSB-EP435322GEN
CSB-BP435322GEN
CSB-MP435322GEN
CSB-EP435322GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP435323GEN
CSB-EP435323GEN
CSB-BP435323GEN
CSB-MP435323GEN
CSB-EP435323GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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