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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Trichoplax adhaerens CDGSH iron-sulfur domain-containing protein 2 homolog (TRIADDRAFT_21706), partial CSB-YP453638TQD
CSB-EP453638TQD
CSB-BP453638TQD
CSB-MP453638TQD
CSB-EP453638TQD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Lipoyl synthase, mitochondrial (TRIADDRAFT_38449), partial CSB-YP453639TQD
CSB-EP453639TQD
CSB-BP453639TQD
CSB-MP453639TQD
CSB-EP453639TQD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 6 CSB-YP453640ROB
CSB-EP453640ROB
CSB-BP453640ROB
CSB-MP453640ROB
CSB-EP453640ROB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 5 CSB-YP453641RNV
CSB-EP453641RNV
CSB-BP453641RNV
CSB-MP453641RNV
CSB-EP453641RNV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP453642ROL
CSB-EP453642ROL
CSB-BP453642ROL
CSB-MP453642ROL
CSB-EP453642ROL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 6 CSB-YP453643ROH
CSB-EP453643ROH
CSB-BP453643ROH
CSB-MP453643ROH
CSB-EP453643ROH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 5 CSB-YP453644RNW
CSB-EP453644RNW
CSB-BP453644RNW
CSB-MP453644RNW
CSB-EP453644RNW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nosema bombycis Spore wall protein 7 (SWP7) CSB-YP453645NHN
CSB-EP453645NHN
CSB-BP453645NHN
CSB-MP453645NHN
CSB-EP453645NHN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP453646BVS1
CSB-EP453646BVS1
CSB-BP453646BVS1
CSB-MP453646BVS1
CSB-EP453646BVS1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon Photosystem II reaction center protein L (psbL), partial CSB-YP453647BVS
CSB-EP453647BVS
CSB-BP453647BVS
CSB-MP453647BVS
CSB-EP453647BVS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP453648BVS
CSB-EP453648BVS
CSB-BP453648BVS
CSB-MP453648BVS
CSB-EP453648BVS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP453649BVS
CSB-EP453649BVS
CSB-BP453649BVS
CSB-MP453649BVS
CSB-EP453649BVS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ourmia melon virus RNA-dependent RNA polymerase, partial CSB-YP453650OFU
CSB-EP453650OFU
CSB-BP453650OFU
CSB-MP453650OFU
CSB-EP453650OFU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana dybowskii Japonicin-1CDYa CSB-YP453651RJK
CSB-EP453651RJK
CSB-BP453651RJK
CSB-MP453651RJK
CSB-EP453651RJK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP453652LMJ
CSB-EP453652LMJ
CSB-BP453652LMJ
CSB-MP453652LMJ
CSB-EP453652LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Adenylosuccinate synthetase (purA) CSB-YP453653LMJ
CSB-EP453653LMJ
CSB-BP453653LMJ
CSB-MP453653LMJ
CSB-EP453653LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Glycogen synthase (glgA) CSB-YP453654LMJ
CSB-EP453654LMJ
CSB-BP453654LMJ
CSB-MP453654LMJ
CSB-EP453654LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Maf-like protein LCABL_24150 (LCABL_24150) CSB-YP453655LMJ
CSB-EP453655LMJ
CSB-BP453655LMJ
CSB-MP453655LMJ
CSB-EP453655LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Initiation-control protein yabA (LCABL_24380) CSB-YP453656LMJ
CSB-EP453656LMJ
CSB-BP453656LMJ
CSB-MP453656LMJ
CSB-EP453656LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L1 (rplA) CSB-YP453657LMJ
CSB-EP453657LMJ
CSB-BP453657LMJ
CSB-MP453657LMJ
CSB-EP453657LMJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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