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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phytoplasma mali Heat-inducible transcription repressor HrcA (hrcA) CSB-YP453598EVH
CSB-EP453598EVH
CSB-BP453598EVH
CSB-MP453598EVH
CSB-EP453598EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L27 (rpmA) CSB-YP453599EVH
CSB-EP453599EVH
CSB-BP453599EVH
CSB-MP453599EVH
CSB-EP453599EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Probable rRNA maturation factor (ATP_00301) CSB-YP453600EVH
CSB-EP453600EVH
CSB-BP453600EVH
CSB-MP453600EVH
CSB-EP453600EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 30S ribosomal protein S17 (rpsQ) CSB-YP453601EVH
CSB-EP453601EVH
CSB-BP453601EVH
CSB-MP453601EVH
CSB-EP453601EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L36 (rpmJ) CSB-YP453602EVH
CSB-EP453602EVH
CSB-BP453602EVH
CSB-MP453602EVH
CSB-EP453602EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali ATP-dependent zinc metalloprotease FtsH 1 (ftsH1), partial CSB-YP453603EVH
CSB-EP453603EVH
CSB-BP453603EVH
CSB-MP453603EVH
CSB-EP453603EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Tyrosine--tRNA ligase (tyrS) CSB-YP453604EVH
CSB-EP453604EVH
CSB-BP453604EVH
CSB-MP453604EVH
CSB-EP453604EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 30S ribosomal protein S20 (rpsT) CSB-YP453605EVH
CSB-EP453605EVH
CSB-BP453605EVH
CSB-MP453605EVH
CSB-EP453605EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Probable cytosol aminopeptidase (pepA) CSB-YP453606EVH
CSB-EP453606EVH
CSB-BP453606EVH
CSB-MP453606EVH
CSB-EP453606EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Tryptophan synthase alpha chain (trpA) CSB-YP453607DZS
CSB-EP453607DZS
CSB-BP453607DZS
CSB-MP453607DZS
CSB-EP453607DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Protein hfq (hfq) CSB-YP453608DZS
CSB-EP453608DZS
CSB-BP453608DZS
CSB-MP453608DZS
CSB-EP453608DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis UPF0133 protein RALTA_A1934 (RALTA_A1934) CSB-YP453609DZS
CSB-EP453609DZS
CSB-BP453609DZS
CSB-MP453609DZS
CSB-EP453609DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Glutamate--tRNA ligase (gltX) CSB-YP453610DZS
CSB-EP453610DZS
CSB-BP453610DZS
CSB-MP453610DZS
CSB-EP453610DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP453611DZS
CSB-EP453611DZS
CSB-BP453611DZS
CSB-MP453611DZS
CSB-EP453611DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP453612DZS
CSB-EP453612DZS
CSB-BP453612DZS
CSB-MP453612DZS
CSB-EP453612DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 50S ribosomal protein L9 (rplI) CSB-YP453613DZS
CSB-EP453613DZS
CSB-BP453613DZS
CSB-MP453613DZS
CSB-EP453613DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 60 kDa chaperonin (groL), partial CSB-YP453614DZS
CSB-EP453614DZS
CSB-BP453614DZS
CSB-MP453614DZS
CSB-EP453614DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 30S ribosomal protein S16 (rpsP) CSB-YP453615DZS
CSB-EP453615DZS
CSB-BP453615DZS
CSB-MP453615DZS
CSB-EP453615DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Ribonuclease PH (rph) CSB-YP453616DZS
CSB-EP453616DZS
CSB-BP453616DZS
CSB-MP453616DZS
CSB-EP453616DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP453617DZS
CSB-EP453617DZS
CSB-BP453617DZS
CSB-MP453617DZS
CSB-EP453617DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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