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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfurihydrogenibium sp. Glycogen synthase (glgA) CSB-YP455038FPP
CSB-EP455038FPP
CSB-BP455038FPP
CSB-MP455038FPP
CSB-EP455038FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP455039FPP
CSB-EP455039FPP
CSB-BP455039FPP
CSB-MP455039FPP
CSB-EP455039FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP455040FPP
CSB-EP455040FPP
CSB-BP455040FPP
CSB-MP455040FPP
CSB-EP455040FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Lon protease (lon), partial CSB-YP455041FPP
CSB-EP455041FPP
CSB-BP455041FPP
CSB-MP455041FPP
CSB-EP455041FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L35 (rpmI) CSB-YP455042FPP
CSB-EP455042FPP
CSB-BP455042FPP
CSB-MP455042FPP
CSB-EP455042FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP455043FPP
CSB-EP455043FPP
CSB-BP455043FPP
CSB-MP455043FPP
CSB-EP455043FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Putative dihydroorotate dehydrogenase A (fumarate) CSB-YP455044FPP
CSB-EP455044FPP
CSB-BP455044FPP
CSB-MP455044FPP
CSB-EP455044FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L13 (rplM) CSB-YP455045FPP
CSB-EP455045FPP
CSB-BP455045FPP
CSB-MP455045FPP
CSB-EP455045FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L36 (rpmJ) CSB-YP455046FPP
CSB-EP455046FPP
CSB-BP455046FPP
CSB-MP455046FPP
CSB-EP455046FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 30S ribosomal protein S8 (rpsH) CSB-YP455047FPP
CSB-EP455047FPP
CSB-BP455047FPP
CSB-MP455047FPP
CSB-EP455047FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L16 (rplP) CSB-YP455048FPP
CSB-EP455048FPP
CSB-BP455048FPP
CSB-MP455048FPP
CSB-EP455048FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP455049FPP
CSB-EP455049FPP
CSB-BP455049FPP
CSB-MP455049FPP
CSB-EP455049FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP455050FPP
CSB-EP455050FPP
CSB-BP455050FPP
CSB-MP455050FPP
CSB-EP455050FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Ribonuclease PH (rph) CSB-YP455051FPP
CSB-EP455051FPP
CSB-BP455051FPP
CSB-MP455051FPP
CSB-EP455051FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Protein grpE (grpE) CSB-YP455052FPP
CSB-EP455052FPP
CSB-BP455052FPP
CSB-MP455052FPP
CSB-EP455052FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. ATP-dependent protease subunit HslV (hslV) CSB-YP455053FPP
CSB-EP455053FPP
CSB-BP455053FPP
CSB-MP455053FPP
CSB-EP455053FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L34 (rpmH) CSB-YP455054FPP
CSB-EP455054FPP
CSB-BP455054FPP
CSB-MP455054FPP
CSB-EP455054FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Bifunctional protein FolD (folD) CSB-YP455055FPP
CSB-EP455055FPP
CSB-BP455055FPP
CSB-MP455055FPP
CSB-EP455055FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP455056ENB1
CSB-EP455056ENB1
CSB-BP455056ENB1
CSB-MP455056ENB1
CSB-EP455056ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP455057ENB
CSB-EP455057ENB
CSB-BP455057ENB
CSB-MP455057ENB
CSB-EP455057ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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