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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Polynucleobacter necessarius Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP453978EXM
CSB-EP453978EXM
CSB-BP453978EXM
CSB-MP453978EXM
CSB-EP453978EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP453979EXM
CSB-EP453979EXM
CSB-BP453979EXM
CSB-MP453979EXM
CSB-EP453979EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L21 (rplU) CSB-YP453980EXM
CSB-EP453980EXM
CSB-BP453980EXM
CSB-MP453980EXM
CSB-EP453980EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Leucine--tRNA ligase (leuS), partial CSB-YP453981EXM
CSB-EP453981EXM
CSB-BP453981EXM
CSB-MP453981EXM
CSB-EP453981EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius UPF0434 protein Pnec_0311 (Pnec_0311) CSB-YP453982EXM
CSB-EP453982EXM
CSB-BP453982EXM
CSB-MP453982EXM
CSB-EP453982EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Elongation factor 4 (lepA), partial CSB-YP453983EXM
CSB-EP453983EXM
CSB-BP453983EXM
CSB-MP453983EXM
CSB-EP453983EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP453984EXM
CSB-EP453984EXM
CSB-BP453984EXM
CSB-MP453984EXM
CSB-EP453984EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribonuclease HII (rnhB) CSB-YP453985EXM
CSB-EP453985EXM
CSB-BP453985EXM
CSB-MP453985EXM
CSB-EP453985EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius GTPase Der (der) CSB-YP453986EXM
CSB-EP453986EXM
CSB-BP453986EXM
CSB-MP453986EXM
CSB-EP453986EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Carbamoyl-phosphate synthase small chain (carA) CSB-YP453987EXM
CSB-EP453987EXM
CSB-BP453987EXM
CSB-MP453987EXM
CSB-EP453987EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Trigger factor (tig) CSB-YP453988EXM
CSB-EP453988EXM
CSB-BP453988EXM
CSB-MP453988EXM
CSB-EP453988EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Tryptophan synthase alpha chain (trpA) CSB-YP453989EXM
CSB-EP453989EXM
CSB-BP453989EXM
CSB-MP453989EXM
CSB-EP453989EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribosome maturation factor RimP (rimP) CSB-YP453990EXM
CSB-EP453990EXM
CSB-BP453990EXM
CSB-MP453990EXM
CSB-EP453990EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP453991EXM
CSB-EP453991EXM
CSB-BP453991EXM
CSB-MP453991EXM
CSB-EP453991EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Cytidylate kinase (cmk) CSB-YP453992EXM
CSB-EP453992EXM
CSB-BP453992EXM
CSB-MP453992EXM
CSB-EP453992EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP453993EXM
CSB-EP453993EXM
CSB-BP453993EXM
CSB-MP453993EXM
CSB-EP453993EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP453994LOT1
CSB-EP453994LOT1
CSB-BP453994LOT1
CSB-MP453994LOT1
CSB-EP453994LOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP453995LOT
CSB-EP453995LOT
CSB-BP453995LOT
CSB-MP453995LOT
CSB-EP453995LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S18 (rpsR) CSB-YP453996LOT
CSB-EP453996LOT
CSB-BP453996LOT
CSB-MP453996LOT
CSB-EP453996LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Protein hfq (hfq) CSB-YP453997LOT
CSB-EP453997LOT
CSB-BP453997LOT
CSB-MP453997LOT
CSB-EP453997LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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