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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit K CSB-YP453958FQA
CSB-EP453958FQA
CSB-BP453958FQA
CSB-MP453958FQA
CSB-EP453958FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L9 (rplI) CSB-YP453959FQA
CSB-EP453959FQA
CSB-BP453959FQA
CSB-MP453959FQA
CSB-EP453959FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cysteine--tRNA ligase (cysS) CSB-YP453960FQA
CSB-EP453960FQA
CSB-BP453960FQA
CSB-MP453960FQA
CSB-EP453960FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I assembly protein ycf3 (ycf3) CSB-YP453961FQA
CSB-EP453961FQA
CSB-BP453961FQA
CSB-MP453961FQA
CSB-EP453961FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable transcriptional regulatory protein SYNPCC7002_A1640 (SYNPCC7002_A1640) CSB-YP453962FQA
CSB-EP453962FQA
CSB-BP453962FQA
CSB-MP453962FQA
CSB-EP453962FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. S-adenosylmethionine synthase (metK) CSB-YP453963FQA
CSB-EP453963FQA
CSB-BP453963FQA
CSB-MP453963FQA
CSB-EP453963FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable rRNA maturation factor (SYNPCC7002_A1764) CSB-YP453964FQA
CSB-EP453964FQA
CSB-BP453964FQA
CSB-MP453964FQA
CSB-EP453964FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate racemase (murI) CSB-YP453965FQA
CSB-EP453965FQA
CSB-BP453965FQA
CSB-MP453965FQA
CSB-EP453965FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP453966FQA
CSB-EP453966FQA
CSB-BP453966FQA
CSB-MP453966FQA
CSB-EP453966FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP453967FQA
CSB-EP453967FQA
CSB-BP453967FQA
CSB-MP453967FQA
CSB-EP453967FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit a 2 (atpB2), partial CSB-YP453968FQA1
CSB-EP453968FQA1
CSB-BP453968FQA1
CSB-MP453968FQA1
CSB-EP453968FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 60 kDa chaperonin (groL), partial CSB-YP453969EXM
CSB-EP453969EXM
CSB-BP453969EXM
CSB-MP453969EXM
CSB-EP453969EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP453970EXM
CSB-EP453970EXM
CSB-BP453970EXM
CSB-MP453970EXM
CSB-EP453970EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Phosphopantetheine adenylyltransferase (coaD) CSB-YP453971EXM
CSB-EP453971EXM
CSB-BP453971EXM
CSB-MP453971EXM
CSB-EP453971EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L11 (rplK) CSB-YP453972EXM
CSB-EP453972EXM
CSB-BP453972EXM
CSB-MP453972EXM
CSB-EP453972EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Acetylglutamate kinase (argB) CSB-YP453973EXM
CSB-EP453973EXM
CSB-BP453973EXM
CSB-MP453973EXM
CSB-EP453973EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP453974EXM
CSB-EP453974EXM
CSB-BP453974EXM
CSB-MP453974EXM
CSB-EP453974EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L23 (rplW) CSB-YP453975EXM
CSB-EP453975EXM
CSB-BP453975EXM
CSB-MP453975EXM
CSB-EP453975EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L24 (rplX) CSB-YP453976EXM
CSB-EP453976EXM
CSB-BP453976EXM
CSB-MP453976EXM
CSB-EP453976EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L36 (rpmJ) CSB-YP453977EXM
CSB-EP453977EXM
CSB-BP453977EXM
CSB-MP453977EXM
CSB-EP453977EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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