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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acinetobacter baumannii Sec-independent protein translocase protein TatA (tatA), partial CSB-YP456299AWO
CSB-EP456299AWO
CSB-BP456299AWO
CSB-MP456299AWO
CSB-EP456299AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Histidine--tRNA ligase (hisS) CSB-YP456300AWO
CSB-EP456300AWO
CSB-BP456300AWO
CSB-MP456300AWO
CSB-EP456300AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP456301XBN
CSB-EP456301XBN
CSB-BP456301XBN
CSB-MP456301XBN
CSB-EP456301XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Aspartate--tRNA ligase (aspS), partial CSB-YP456302XBN
CSB-EP456302XBN
CSB-BP456302XBN
CSB-MP456302XBN
CSB-EP456302XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP456303XBN
CSB-EP456303XBN
CSB-BP456303XBN
CSB-MP456303XBN
CSB-EP456303XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Acireductone dioxygenase (mtnD) CSB-YP456304XBN
CSB-EP456304XBN
CSB-BP456304XBN
CSB-MP456304XBN
CSB-EP456304XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Histidine--tRNA ligase (hisS) CSB-YP456305XBN
CSB-EP456305XBN
CSB-BP456305XBN
CSB-MP456305XBN
CSB-EP456305XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0059 membrane protein XfasM23_1382 (XfasM23_1382), partial CSB-YP456306XBN1
CSB-EP456306XBN1
CSB-BP456306XBN1
CSB-MP456306XBN1
CSB-EP456306XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Biotin synthase (bioB) CSB-YP456307XBN
CSB-EP456307XBN
CSB-BP456307XBN
CSB-MP456307XBN
CSB-EP456307XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Dihydroxy-acid dehydratase (ilvD), partial CSB-YP456308XBN
CSB-EP456308XBN
CSB-BP456308XBN
CSB-MP456308XBN
CSB-EP456308XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Heat-inducible transcription repressor HrcA (hrcA) CSB-YP456309XBN
CSB-EP456309XBN
CSB-BP456309XBN
CSB-MP456309XBN
CSB-EP456309XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cytidylate kinase (cmk) CSB-YP456310XBN
CSB-EP456310XBN
CSB-BP456310XBN
CSB-MP456310XBN
CSB-EP456310XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP456311XBN
CSB-EP456311XBN
CSB-BP456311XBN
CSB-MP456311XBN
CSB-EP456311XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP456312XBN
CSB-EP456312XBN
CSB-BP456312XBN
CSB-MP456312XBN
CSB-EP456312XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP456313XBN
CSB-EP456313XBN
CSB-BP456313XBN
CSB-MP456313XBN
CSB-EP456313XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP456314XBN
CSB-EP456314XBN
CSB-BP456314XBN
CSB-MP456314XBN
CSB-EP456314XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP456315XBN
CSB-EP456315XBN
CSB-BP456315XBN
CSB-MP456315XBN
CSB-EP456315XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L4 (rplD) CSB-YP456316XBN
CSB-EP456316XBN
CSB-BP456316XBN
CSB-MP456316XBN
CSB-EP456316XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S17 (rpsQ) CSB-YP456317XBN
CSB-EP456317XBN
CSB-BP456317XBN
CSB-MP456317XBN
CSB-EP456317XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L6 (rplF) CSB-YP456318XBN
CSB-EP456318XBN
CSB-BP456318XBN
CSB-MP456318XBN
CSB-EP456318XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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