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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus fermentum Bifunctional protein pyrR (pyrR) CSB-YP456219LMO
CSB-EP456219LMO
CSB-BP456219LMO
CSB-MP456219LMO
CSB-EP456219LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Tyrosine--tRNA ligase (tyrS) CSB-YP456220LMO
CSB-EP456220LMO
CSB-BP456220LMO
CSB-MP456220LMO
CSB-EP456220LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP456221KBO
CSB-EP456221KBO
CSB-BP456221KBO
CSB-MP456221KBO
CSB-EP456221KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Uracil phosphoribosyltransferase (upp) CSB-YP456222KBO
CSB-EP456222KBO
CSB-BP456222KBO
CSB-MP456222KBO
CSB-EP456222KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP456223KBO
CSB-EP456223KBO
CSB-BP456223KBO
CSB-MP456223KBO
CSB-EP456223KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Deoxycytidine triphosphate deaminase (dcd) CSB-YP456224KBO
CSB-EP456224KBO
CSB-BP456224KBO
CSB-MP456224KBO
CSB-EP456224KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L27 (rpmA) CSB-YP456225KBO
CSB-EP456225KBO
CSB-BP456225KBO
CSB-MP456225KBO
CSB-EP456225KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP456226KBO
CSB-EP456226KBO
CSB-BP456226KBO
CSB-MP456226KBO
CSB-EP456226KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Urease accessory protein UreG (ureG) CSB-YP456227KBO
CSB-EP456227KBO
CSB-BP456227KBO
CSB-MP456227KBO
CSB-EP456227KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Protease HtpX homolog (htpX), partial CSB-YP456228KBO1
CSB-EP456228KBO1
CSB-BP456228KBO1
CSB-MP456228KBO1
CSB-EP456228KBO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L11 (rplK) CSB-YP456229KBO
CSB-EP456229KBO
CSB-BP456229KBO
CSB-MP456229KBO
CSB-EP456229KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP456230KBO
CSB-EP456230KBO
CSB-BP456230KBO
CSB-MP456230KBO
CSB-EP456230KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila AAA ATPase forming ring-shaped complexes (arc), partial CSB-YP456231KBO
CSB-EP456231KBO
CSB-BP456231KBO
CSB-MP456231KBO
CSB-EP456231KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L14 (rplN) CSB-YP456232KBO
CSB-EP456232KBO
CSB-BP456232KBO
CSB-MP456232KBO
CSB-EP456232KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP456233KBO
CSB-EP456233KBO
CSB-BP456233KBO
CSB-MP456233KBO
CSB-EP456233KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP456234KBO
CSB-EP456234KBO
CSB-BP456234KBO
CSB-MP456234KBO
CSB-EP456234KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L34 (rpmH) CSB-YP456235KBO
CSB-EP456235KBO
CSB-BP456235KBO
CSB-MP456235KBO
CSB-EP456235KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L20 (rplT) CSB-YP456236KBO
CSB-EP456236KBO
CSB-BP456236KBO
CSB-MP456236KBO
CSB-EP456236KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Glutamyl-tRNA reductase (hemA) CSB-YP456237KBO
CSB-EP456237KBO
CSB-BP456237KBO
CSB-MP456237KBO
CSB-EP456237KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Dihydroxy-acid dehydratase (ilvD), partial CSB-YP456238KBO
CSB-EP456238KBO
CSB-BP456238KBO
CSB-MP456238KBO
CSB-EP456238KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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