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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP456319XBN
CSB-EP456319XBN
CSB-BP456319XBN
CSB-MP456319XBN
CSB-EP456319XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa D-amino acid dehydrogenase small subunit (dadA) CSB-YP456320XBN
CSB-EP456320XBN
CSB-BP456320XBN
CSB-MP456320XBN
CSB-EP456320XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cell division protein FtsB (ftsB), partial CSB-YP456321XBN
CSB-EP456321XBN
CSB-BP456321XBN
CSB-MP456321XBN
CSB-EP456321XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP456322XBN
CSB-EP456322XBN
CSB-BP456322XBN
CSB-MP456322XBN
CSB-EP456322XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP456323XBN
CSB-EP456323XBN
CSB-BP456323XBN
CSB-MP456323XBN
CSB-EP456323XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP456324XBN
CSB-EP456324XBN
CSB-BP456324XBN
CSB-MP456324XBN
CSB-EP456324XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S2 (rpsB) CSB-YP456325XBN
CSB-EP456325XBN
CSB-BP456325XBN
CSB-MP456325XBN
CSB-EP456325XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP456326XBN
CSB-EP456326XBN
CSB-BP456326XBN
CSB-MP456326XBN
CSB-EP456326XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP456327BSP
CSB-EP456327BSP
CSB-BP456327BSP
CSB-MP456327BSP
CSB-EP456327BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Chaperone protein DnaK (dnaK), partial CSB-YP456328BSP
CSB-EP456328BSP
CSB-BP456328BSP
CSB-MP456328BSP
CSB-EP456328BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP456329BSP
CSB-EP456329BSP
CSB-BP456329BSP
CSB-MP456329BSP
CSB-EP456329BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica UPF0133 protein Bind_0255 (Bind_0255) CSB-YP456330BSP
CSB-EP456330BSP
CSB-BP456330BSP
CSB-MP456330BSP
CSB-EP456330BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP456331BSP
CSB-EP456331BSP
CSB-BP456331BSP
CSB-MP456331BSP
CSB-EP456331BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Histidinol-phosphate aminotransferase (hisC) CSB-YP456332BSP
CSB-EP456332BSP
CSB-BP456332BSP
CSB-MP456332BSP
CSB-EP456332BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Membrane protein insertase YidC (yidC), partial CSB-YP456333BSP1
CSB-EP456333BSP1
CSB-BP456333BSP1
CSB-MP456333BSP1
CSB-EP456333BSP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP456334BSP
CSB-EP456334BSP
CSB-BP456334BSP
CSB-MP456334BSP
CSB-EP456334BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP456335BSP
CSB-EP456335BSP
CSB-BP456335BSP
CSB-MP456335BSP
CSB-EP456335BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Phosphate acyltransferase (plsX) CSB-YP456336BSP
CSB-EP456336BSP
CSB-BP456336BSP
CSB-MP456336BSP
CSB-EP456336BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP456337BSP
CSB-EP456337BSP
CSB-BP456337BSP
CSB-MP456337BSP
CSB-EP456337BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Peptidyl-tRNA hydrolase (pth) CSB-YP456338BSP
CSB-EP456338BSP
CSB-BP456338BSP
CSB-MP456338BSP
CSB-EP456338BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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