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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nostoc punctiforme tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP456400NHQ
CSB-EP456400NHQ
CSB-BP456400NHQ
CSB-MP456400NHQ
CSB-EP456400NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP456401NHQ1
CSB-EP456401NHQ1
CSB-BP456401NHQ1
CSB-MP456401NHQ1
CSB-EP456401NHQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Arginine--tRNA ligase (argS), partial CSB-YP456402NHQ
CSB-EP456402NHQ
CSB-BP456402NHQ
CSB-MP456402NHQ
CSB-EP456402NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit J CSB-YP456403NHQ
CSB-EP456403NHQ
CSB-BP456403NHQ
CSB-MP456403NHQ
CSB-EP456403NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem II reaction center protein J (psbJ), partial CSB-YP456404NHQ
CSB-EP456404NHQ
CSB-BP456404NHQ
CSB-MP456404NHQ
CSB-EP456404NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UPF0133 protein Npun_F0448 (Npun_F0448) CSB-YP456405NHQ
CSB-EP456405NHQ
CSB-BP456405NHQ
CSB-MP456405NHQ
CSB-EP456405NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Leucine--tRNA ligase (leuS), partial CSB-YP456406NHQ
CSB-EP456406NHQ
CSB-BP456406NHQ
CSB-MP456406NHQ
CSB-EP456406NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP456407NHQ
CSB-EP456407NHQ
CSB-BP456407NHQ
CSB-MP456407NHQ
CSB-EP456407NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 30S ribosomal protein S15 (rpsO) CSB-YP456408NHQ
CSB-EP456408NHQ
CSB-BP456408NHQ
CSB-MP456408NHQ
CSB-EP456408NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Aspartate 1-decarboxylase (panD) CSB-YP456409NHQ
CSB-EP456409NHQ
CSB-BP456409NHQ
CSB-MP456409NHQ
CSB-EP456409NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum C4-dicarboxylate transport protein (dctA), partial CSB-YP456410BXT
CSB-EP456410BXT
CSB-BP456410BXT
CSB-MP456410BXT
CSB-EP456410BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum N-succinylarginine dihydrolase (astB) CSB-YP456411BXT
CSB-EP456411BXT
CSB-BP456411BXT
CSB-MP456411BXT
CSB-EP456411BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0042 nucleotide-binding protein Bphy_0322 (Bphy_0322) CSB-YP456412BXT
CSB-EP456412BXT
CSB-BP456412BXT
CSB-MP456412BXT
CSB-EP456412BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP456413BXT
CSB-EP456413BXT
CSB-BP456413BXT
CSB-MP456413BXT
CSB-EP456413BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP456414BXT
CSB-EP456414BXT
CSB-BP456414BXT
CSB-MP456414BXT
CSB-EP456414BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP456415BXT
CSB-EP456415BXT
CSB-BP456415BXT
CSB-MP456415BXT
CSB-EP456415BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0434 protein Bphy_0537 (Bphy_0537) CSB-YP456416BXT
CSB-EP456416BXT
CSB-BP456416BXT
CSB-MP456416BXT
CSB-EP456416BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Quinolinate synthase A (nadA) CSB-YP456417BXT
CSB-EP456417BXT
CSB-BP456417BXT
CSB-MP456417BXT
CSB-EP456417BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP456418BXT
CSB-EP456418BXT
CSB-BP456418BXT
CSB-MP456418BXT
CSB-EP456418BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP456419BXT1
CSB-EP456419BXT1
CSB-BP456419BXT1
CSB-MP456419BXT1
CSB-EP456419BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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