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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Chromosome initiation inhibitor (iciA) CSB-YP456460YAW
CSB-EP456460YAW
CSB-BP456460YAW
CSB-MP456460YAW
CSB-EP456460YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP456461YAW
CSB-EP456461YAW
CSB-BP456461YAW
CSB-MP456461YAW
CSB-EP456461YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L10 (rplJ) CSB-YP456462YAW
CSB-EP456462YAW
CSB-BP456462YAW
CSB-MP456462YAW
CSB-EP456462YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB LexA repressor (lexA) CSB-YP456463YAW
CSB-EP456463YAW
CSB-BP456463YAW
CSB-MP456463YAW
CSB-EP456463YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 10 kDa chaperonin (groS) CSB-YP456464YAW
CSB-EP456464YAW
CSB-BP456464YAW
CSB-MP456464YAW
CSB-EP456464YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Bifunctional protein HldE (hldE) CSB-YP456465YAW
CSB-EP456465YAW
CSB-BP456465YAW
CSB-MP456465YAW
CSB-EP456465YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S6 (rpsF) CSB-YP456466YAW
CSB-EP456466YAW
CSB-BP456466YAW
CSB-MP456466YAW
CSB-EP456466YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L21 (rplU) CSB-YP456467YAW
CSB-EP456467YAW
CSB-BP456467YAW
CSB-MP456467YAW
CSB-EP456467YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S15 (rpsO) CSB-YP456468YAW
CSB-EP456468YAW
CSB-BP456468YAW
CSB-MP456468YAW
CSB-EP456468YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable phosphatase YPTS_2077 (YPTS_2077) CSB-YP456469YAW
CSB-EP456469YAW
CSB-BP456469YAW
CSB-MP456469YAW
CSB-EP456469YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA (cmo5U34)-methyltransferase 2 CSB-YP456470YAW
CSB-EP456470YAW
CSB-BP456470YAW
CSB-MP456470YAW
CSB-EP456470YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP456471YAW
CSB-EP456471YAW
CSB-BP456471YAW
CSB-MP456471YAW
CSB-EP456471YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP456472YAW
CSB-EP456472YAW
CSB-BP456472YAW
CSB-MP456472YAW
CSB-EP456472YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Homoserine kinase (thrB) CSB-YP456473YAW
CSB-EP456473YAW
CSB-BP456473YAW
CSB-MP456473YAW
CSB-EP456473YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP456474YAW
CSB-EP456474YAW
CSB-BP456474YAW
CSB-MP456474YAW
CSB-EP456474YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0260 protein YPTS_2126 (YPTS_2126) CSB-YP456475YAW
CSB-EP456475YAW
CSB-BP456475YAW
CSB-MP456475YAW
CSB-EP456475YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0225 protein YPTS_2164 (YPTS_2164) CSB-YP456476YAW
CSB-EP456476YAW
CSB-BP456476YAW
CSB-MP456476YAW
CSB-EP456476YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein AaeX (aaeX), partial CSB-YP456477YAW1
CSB-EP456477YAW1
CSB-BP456477YAW1
CSB-MP456477YAW1
CSB-EP456477YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB RNA polymerase-associated protein RapA (rapA), partial CSB-YP456478YAW
CSB-EP456478YAW
CSB-BP456478YAW
CSB-MP456478YAW
CSB-EP456478YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 2-isopropylmalate synthase (leuA), partial CSB-YP456479YAW
CSB-EP456479YAW
CSB-BP456479YAW
CSB-MP456479YAW
CSB-EP456479YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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