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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Ketol-acid reductoisomerase (ilvC) CSB-YP456660XBC
CSB-EP456660XBC
CSB-BP456660XBC
CSB-MP456660XBC
CSB-EP456660XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP456661XBC
CSB-EP456661XBC
CSB-BP456661XBC
CSB-MP456661XBC
CSB-EP456661XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Proline--tRNA ligase (proS), partial CSB-YP456662XBC
CSB-EP456662XBC
CSB-BP456662XBC
CSB-MP456662XBC
CSB-EP456662XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP456663XBC
CSB-EP456663XBC
CSB-BP456663XBC
CSB-MP456663XBC
CSB-EP456663XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae ATP synthase gamma chain (atpG) CSB-YP456664XBC
CSB-EP456664XBC
CSB-BP456664XBC
CSB-MP456664XBC
CSB-EP456664XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L10 (rplJ) CSB-YP456665XBC
CSB-EP456665XBC
CSB-BP456665XBC
CSB-MP456665XBC
CSB-EP456665XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L23 (rplW) CSB-YP456666XBC
CSB-EP456666XBC
CSB-BP456666XBC
CSB-MP456666XBC
CSB-EP456666XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L24 (rplX) CSB-YP456667XBC
CSB-EP456667XBC
CSB-BP456667XBC
CSB-MP456667XBC
CSB-EP456667XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 30S ribosomal protein S4 (rpsD) CSB-YP456668XBC
CSB-EP456668XBC
CSB-BP456668XBC
CSB-MP456668XBC
CSB-EP456668XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP456669XBC
CSB-EP456669XBC
CSB-BP456669XBC
CSB-MP456669XBC
CSB-EP456669XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae DNA repair protein recO (recO) CSB-YP456670XBC
CSB-EP456670XBC
CSB-BP456670XBC
CSB-MP456670XBC
CSB-EP456670XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Glutamate--tRNA ligase (gltX) CSB-YP456671XBC
CSB-EP456671XBC
CSB-BP456671XBC
CSB-MP456671XBC
CSB-EP456671XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L27 (rpmA) CSB-YP456672XBC
CSB-EP456672XBC
CSB-BP456672XBC
CSB-MP456672XBC
CSB-EP456672XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Lipoprotein signal peptidase (lspA), partial CSB-YP456673XBC1
CSB-EP456673XBC1
CSB-BP456673XBC1
CSB-MP456673XBC1
CSB-EP456673XBC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP456674XBC
CSB-EP456674XBC
CSB-BP456674XBC
CSB-MP456674XBC
CSB-EP456674XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 5'-nucleotidase surE (surE) CSB-YP456675XBC
CSB-EP456675XBC
CSB-BP456675XBC
CSB-MP456675XBC
CSB-EP456675XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP456676XBC
CSB-EP456676XBC
CSB-BP456676XBC
CSB-MP456676XBC
CSB-EP456676XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP456677XBC
CSB-EP456677XBC
CSB-BP456677XBC
CSB-MP456677XBC
CSB-EP456677XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Biotin synthase (bioB) CSB-YP456678BXU
CSB-EP456678BXU
CSB-BP456678BXU
CSB-MP456678BXU
CSB-EP456678BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP456679BXU
CSB-EP456679BXU
CSB-BP456679BXU
CSB-MP456679BXU
CSB-EP456679BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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