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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila yakuba Lipoyl synthase 1, mitochondrial (Las1), partial CSB-YP457661DMR
CSB-EP457661DMR
CSB-BP457661DMR
CSB-MP457661DMR
CSB-EP457661DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein cueball (cue), partial CSB-YP457662DLL
CSB-EP457662DLL
CSB-BP457662DLL
CSB-MP457662DLL
CSB-EP457662DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi WD repeat-containing protein 48 homolog (GH21936), partial CSB-YP457663DLL
CSB-EP457663DLL
CSB-BP457663DLL
CSB-MP457663DLL
CSB-EP457663DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Werner Syndrome-like exonuclease (WRNexo) CSB-YP457664DLL
CSB-EP457664DLL
CSB-BP457664DLL
CSB-MP457664DLL
CSB-EP457664DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Regulator of telomere elongation helicase 1 homolog (GH24089), partial CSB-YP457665DLL
CSB-EP457665DLL
CSB-BP457665DLL
CSB-MP457665DLL
CSB-EP457665DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Microtubule-associated protein Jupiter (Jupiter) CSB-YP457666DLL
CSB-EP457666DLL
CSB-BP457666DLL
CSB-MP457666DLL
CSB-EP457666DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein CLP1 homolog (cbc) CSB-YP457667DLL
CSB-EP457667DLL
CSB-BP457667DLL
CSB-MP457667DLL
CSB-EP457667DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein crossbronx-like (GH22748) CSB-YP457668DLL
CSB-EP457668DLL
CSB-BP457668DLL
CSB-MP457668DLL
CSB-EP457668DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP457669DLL
CSB-EP457669DLL
CSB-BP457669DLL
CSB-MP457669DLL
CSB-EP457669DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Transmembrane emp24 domain-containing protein bai (bai), partial CSB-YP457670DLL
CSB-EP457670DLL
CSB-BP457670DLL
CSB-MP457670DLL
CSB-EP457670DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein hedgehog (hh) CSB-YP457671DLY
CSB-EP457671DLY
CSB-BP457671DLY
CSB-MP457671DLY
CSB-EP457671DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis CDGSH iron-sulfur domain-containing protein 2 homolog (GI10402), partial CSB-YP457672DLY
CSB-EP457672DLY
CSB-BP457672DLY
CSB-MP457672DLY
CSB-EP457672DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP457673DLY1
CSB-EP457673DLY1
CSB-BP457673DLY1
CSB-MP457673DLY1
CSB-EP457673DLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP457674DLY
CSB-EP457674DLY
CSB-BP457674DLY
CSB-MP457674DLY
CSB-EP457674DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Microtubule-associated protein Jupiter (Jupiter) CSB-YP457675DLY
CSB-EP457675DLY
CSB-BP457675DLY
CSB-MP457675DLY
CSB-EP457675DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein hook (hk), partial CSB-YP457676DLY
CSB-EP457676DLY
CSB-BP457676DLY
CSB-MP457676DLY
CSB-EP457676DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Probable cytosolic Fe-S cluster assembly factor GI11683 (GI11683) CSB-YP457677DLY
CSB-EP457677DLY
CSB-BP457677DLY
CSB-MP457677DLY
CSB-EP457677DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Cytosolic Fe-S cluster assembly factor NUBP1 homolog (GI17051) CSB-YP457678DLY
CSB-EP457678DLY
CSB-BP457678DLY
CSB-MP457678DLY
CSB-EP457678DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Integrator complex subunit 3 homolog (IntS3), partial CSB-YP457679DLY
CSB-EP457679DLY
CSB-BP457679DLY
CSB-MP457679DLY
CSB-EP457679DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 homolog (GI16965), partial CSB-YP457680DLY
CSB-EP457680DLY
CSB-BP457680DLY
CSB-MP457680DLY
CSB-EP457680DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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