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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phytofirmans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP456680BXU
CSB-EP456680BXU
CSB-BP456680BXU
CSB-MP456680BXU
CSB-EP456680BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0042 nucleotide-binding protein Bphyt_0592 (Bphyt_0592) CSB-YP456681BXU
CSB-EP456681BXU
CSB-BP456681BXU
CSB-MP456681BXU
CSB-EP456681BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP456682BXU
CSB-EP456682BXU
CSB-BP456682BXU
CSB-MP456682BXU
CSB-EP456682BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Lysine--tRNA ligase (lysS), partial CSB-YP456683BXU
CSB-EP456683BXU
CSB-BP456683BXU
CSB-MP456683BXU
CSB-EP456683BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP456684BXU
CSB-EP456684BXU
CSB-BP456684BXU
CSB-MP456684BXU
CSB-EP456684BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP456685BXU
CSB-EP456685BXU
CSB-BP456685BXU
CSB-MP456685BXU
CSB-EP456685BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans GTPase obg (obg) CSB-YP456686BXU
CSB-EP456686BXU
CSB-BP456686BXU
CSB-MP456686BXU
CSB-EP456686BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP456687BXU
CSB-EP456687BXU
CSB-BP456687BXU
CSB-MP456687BXU
CSB-EP456687BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glutamyl-tRNA reductase (hemA) CSB-YP456688BXU
CSB-EP456688BXU
CSB-BP456688BXU
CSB-MP456688BXU
CSB-EP456688BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP456689BXU
CSB-EP456689BXU
CSB-BP456689BXU
CSB-MP456689BXU
CSB-EP456689BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Elongation factor P (efp) CSB-YP456690BXU
CSB-EP456690BXU
CSB-BP456690BXU
CSB-MP456690BXU
CSB-EP456690BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Transcriptional repressor NrdR (nrdR) CSB-YP456691BXU
CSB-EP456691BXU
CSB-BP456691BXU
CSB-MP456691BXU
CSB-EP456691BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Cobyric acid synthase (cobQ) CSB-YP456692BXU
CSB-EP456692BXU
CSB-BP456692BXU
CSB-MP456692BXU
CSB-EP456692BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Acetylglutamate kinase (argB) CSB-YP456693BXU
CSB-EP456693BXU
CSB-BP456693BXU
CSB-MP456693BXU
CSB-EP456693BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Uracil phosphoribosyltransferase (upp) CSB-YP456694BXU
CSB-EP456694BXU
CSB-BP456694BXU
CSB-MP456694BXU
CSB-EP456694BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP456695BXU
CSB-EP456695BXU
CSB-BP456695BXU
CSB-MP456695BXU
CSB-EP456695BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 4-hydroxy-2-oxovalerate aldolase (Bphyt_1468) CSB-YP456696BXU
CSB-EP456696BXU
CSB-BP456696BXU
CSB-MP456696BXU
CSB-EP456696BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Alkanesulfonate monooxygenase (ssuD) CSB-YP456697BXU
CSB-EP456697BXU
CSB-BP456697BXU
CSB-MP456697BXU
CSB-EP456697BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP456698BXU
CSB-EP456698BXU
CSB-BP456698BXU
CSB-MP456698BXU
CSB-EP456698BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative reductase Bphyt_2012 (Bphyt_2012) CSB-YP456699BXU
CSB-EP456699BXU
CSB-BP456699BXU
CSB-MP456699BXU
CSB-EP456699BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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