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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kocuria rhizophila 50S ribosomal protein L28 (rpmB) CSB-YP458141KBO
CSB-EP458141KBO
CSB-BP458141KBO
CSB-MP458141KBO
CSB-EP458141KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Trigger factor (tig) CSB-YP458142KBO
CSB-EP458142KBO
CSB-BP458142KBO
CSB-MP458142KBO
CSB-EP458142KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila ATP phosphoribosyltransferase (hisG) CSB-YP458143KBO
CSB-EP458143KBO
CSB-BP458143KBO
CSB-MP458143KBO
CSB-EP458143KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Putative sporulation transcription regulator WhiA (whiA) CSB-YP458144KBO
CSB-EP458144KBO
CSB-BP458144KBO
CSB-MP458144KBO
CSB-EP458144KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Urease accessory protein UreD (ureD) CSB-YP458145KBO
CSB-EP458145KBO
CSB-BP458145KBO
CSB-MP458145KBO
CSB-EP458145KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Elongation factor P (efp) CSB-YP458146KBO
CSB-EP458146KBO
CSB-BP458146KBO
CSB-MP458146KBO
CSB-EP458146KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L7/L12 (rplL) CSB-YP458147KBO
CSB-EP458147KBO
CSB-BP458147KBO
CSB-MP458147KBO
CSB-EP458147KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S10 (rpsJ) CSB-YP458148KBO
CSB-EP458148KBO
CSB-BP458148KBO
CSB-MP458148KBO
CSB-EP458148KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP458149KBO
CSB-EP458149KBO
CSB-BP458149KBO
CSB-MP458149KBO
CSB-EP458149KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L16 (rplP) CSB-YP458150KBO
CSB-EP458150KBO
CSB-BP458150KBO
CSB-MP458150KBO
CSB-EP458150KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Adenylate kinase (adk) CSB-YP458151KBO
CSB-EP458151KBO
CSB-BP458151KBO
CSB-MP458151KBO
CSB-EP458151KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP458152KBO
CSB-EP458152KBO
CSB-BP458152KBO
CSB-MP458152KBO
CSB-EP458152KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Ribonuclease P protein component (rnpA) CSB-YP458153KBO
CSB-EP458153KBO
CSB-BP458153KBO
CSB-MP458153KBO
CSB-EP458153KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Ribosome-binding factor A (rbfA) CSB-YP458154KBO
CSB-EP458154KBO
CSB-BP458154KBO
CSB-MP458154KBO
CSB-EP458154KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Proline--tRNA ligase (proS), partial CSB-YP458155KBO
CSB-EP458155KBO
CSB-BP458155KBO
CSB-MP458155KBO
CSB-EP458155KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L25 (rplY) CSB-YP458156KBO
CSB-EP458156KBO
CSB-BP458156KBO
CSB-MP458156KBO
CSB-EP458156KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila ATP synthase gamma chain (atpG) CSB-YP458157KBO
CSB-EP458157KBO
CSB-BP458157KBO
CSB-MP458157KBO
CSB-EP458157KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein CXorf57 homolog, partial CSB-YP458158RA
CSB-EP458158RA
CSB-BP458158RA
CSB-MP458158RA
CSB-EP458158RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S14 type Z (rpsZ) CSB-YP458159MVS
CSB-EP458159MVS
CSB-BP458159MVS
CSB-MP458159MVS
CSB-EP458159MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_1057 (MMAR_1057) CSB-YP458160MVS
CSB-EP458160MVS
CSB-BP458160MVS
CSB-MP458160MVS
CSB-EP458160MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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