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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia Putative arginyl-tRNA--protein transferase (ate) CSB-YP458082FLX
CSB-EP458082FLX
CSB-BP458082FLX
CSB-MP458082FLX
CSB-EP458082FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Isoleucine--tRNA ligase (ileS), partial CSB-YP458083FLX
CSB-EP458083FLX
CSB-BP458083FLX
CSB-MP458083FLX
CSB-EP458083FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP458084FLX
CSB-EP458084FLX
CSB-BP458084FLX
CSB-MP458084FLX
CSB-EP458084FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heteractis crispa Analgesic polypeptide HC1 CSB-YP458085HWE
CSB-EP458085HWE
CSB-BP458085HWE
CSB-MP458085HWE
CSB-EP458085HWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii S-layer biosynthesis glycosyltransferase AglG (aglG) CSB-YP458086HTT
CSB-EP458086HTT
CSB-BP458086HTT
CSB-MP458086HTT
CSB-EP458086HTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S18 (rpsR) CSB-YP458087LMV
CSB-EP458087LMV
CSB-BP458087LMV
CSB-MP458087LMV
CSB-EP458087LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Serine--tRNA ligase (serS) CSB-YP458088LMV
CSB-EP458088LMV
CSB-BP458088LMV
CSB-MP458088LMV
CSB-EP458088LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Formate--tetrahydrofolate ligase (fhs), partial CSB-YP458089LMV
CSB-EP458089LMV
CSB-BP458089LMV
CSB-MP458089LMV
CSB-EP458089LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Protease HtpX homolog (htpX), partial CSB-YP458090LMV1
CSB-EP458090LMV1
CSB-BP458090LMV1
CSB-MP458090LMV1
CSB-EP458090LMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L33 1 (rpmG1) CSB-YP458091LMV
CSB-EP458091LMV
CSB-BP458091LMV
CSB-MP458091LMV
CSB-EP458091LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L7/L12 (rplL) CSB-YP458092LMV
CSB-EP458092LMV
CSB-BP458092LMV
CSB-MP458092LMV
CSB-EP458092LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UPF0042 nucleotide-binding protein LAR_0375 (LAR_0375) CSB-YP458093LMV
CSB-EP458093LMV
CSB-BP458093LMV
CSB-MP458093LMV
CSB-EP458093LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phosphoglucosamine mutase (glmM) CSB-YP458094LMV
CSB-EP458094LMV
CSB-BP458094LMV
CSB-MP458094LMV
CSB-EP458094LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA mismatch repair protein mutL (mutL), partial CSB-YP458095LMV
CSB-EP458095LMV
CSB-BP458095LMV
CSB-MP458095LMV
CSB-EP458095LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Protein MraZ (mraZ) CSB-YP458096LMV
CSB-EP458096LMV
CSB-BP458096LMV
CSB-MP458096LMV
CSB-EP458096LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Dihydrodipicolinate synthase (dapA) CSB-YP458097LMV
CSB-EP458097LMV
CSB-BP458097LMV
CSB-MP458097LMV
CSB-EP458097LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S15 (rpsO) CSB-YP458098LMV
CSB-EP458098LMV
CSB-BP458098LMV
CSB-MP458098LMV
CSB-EP458098LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri LexA repressor (lexA) CSB-YP458099LMV
CSB-EP458099LMV
CSB-BP458099LMV
CSB-MP458099LMV
CSB-EP458099LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosome-binding factor A (rbfA) CSB-YP458100LMV
CSB-EP458100LMV
CSB-BP458100LMV
CSB-MP458100LMV
CSB-EP458100LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Elongation factor 4 (lepA), partial CSB-YP458101LMV
CSB-EP458101LMV
CSB-BP458101LMV
CSB-MP458101LMV
CSB-EP458101LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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