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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Treponema pallidum subsp. pallidum 30S ribosomal protein S2 (rpsB) CSB-YP458542TPS
CSB-EP458542TPS
CSB-BP458542TPS
CSB-MP458542TPS
CSB-EP458542TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Flagellar assembly factor FliW (fliW) CSB-YP458543TPS
CSB-EP458543TPS
CSB-BP458543TPS
CSB-MP458543TPS
CSB-EP458543TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum GTPase Der (der) CSB-YP458544TPS
CSB-EP458544TPS
CSB-BP458544TPS
CSB-MP458544TPS
CSB-EP458544TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 50S ribosomal protein L27 (rpmA) CSB-YP458545TPS
CSB-EP458545TPS
CSB-BP458545TPS
CSB-MP458545TPS
CSB-EP458545TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 30S ribosomal protein S21 (rpsU) CSB-YP458546TPS
CSB-EP458546TPS
CSB-BP458546TPS
CSB-MP458546TPS
CSB-EP458546TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP458547TPS
CSB-EP458547TPS
CSB-BP458547TPS
CSB-MP458547TPS
CSB-EP458547TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP458548TPS
CSB-EP458548TPS
CSB-BP458548TPS
CSB-MP458548TPS
CSB-EP458548TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Phenylalanine--tRNA ligase alpha subunit (pheS), partial CSB-YP458549TPS
CSB-EP458549TPS
CSB-BP458549TPS
CSB-MP458549TPS
CSB-EP458549TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Glutamyl-tRNA (Gln) amidotransferase subunit A, partial CSB-YP458550TPS
CSB-EP458550TPS
CSB-BP458550TPS
CSB-MP458550TPS
CSB-EP458550TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP458551BWR
CSB-EP458551BWR
CSB-BP458551BWR
CSB-MP458551BWR
CSB-EP458551BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Proline--tRNA ligase (proS) CSB-YP458552BWR
CSB-EP458552BWR
CSB-BP458552BWR
CSB-MP458552BWR
CSB-EP458552BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Cobalamin synthase (cobS), partial CSB-YP458553BWR1
CSB-EP458553BWR1
CSB-BP458553BWR1
CSB-MP458553BWR1
CSB-EP458553BWR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Trigger factor (tig) CSB-YP458554BWR
CSB-EP458554BWR
CSB-BP458554BWR
CSB-MP458554BWR
CSB-EP458554BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UPF0283 membrane protein BAbS19_I09770 (BAbS19_I09770), partial CSB-YP458555BWR1
CSB-EP458555BWR1
CSB-BP458555BWR1
CSB-MP458555BWR1
CSB-EP458555BWR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP458556BWR
CSB-EP458556BWR
CSB-BP458556BWR
CSB-MP458556BWR
CSB-EP458556BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S2 (rpsB) CSB-YP458557BWR
CSB-EP458557BWR
CSB-BP458557BWR
CSB-MP458557BWR
CSB-EP458557BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Protein RecA (recA) CSB-YP458558BWR
CSB-EP458558BWR
CSB-BP458558BWR
CSB-MP458558BWR
CSB-EP458558BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L30 (rpmD) CSB-YP458559BWR
CSB-EP458559BWR
CSB-BP458559BWR
CSB-MP458559BWR
CSB-EP458559BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L6 (rplF) CSB-YP458560BWR
CSB-EP458560BWR
CSB-BP458560BWR
CSB-MP458560BWR
CSB-EP458560BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L14 (rplN) CSB-YP458561BWR
CSB-EP458561BWR
CSB-BP458561BWR
CSB-MP458561BWR
CSB-EP458561BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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