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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_1069 (MMAR_1069) CSB-YP458161MVS
CSB-EP458161MVS
CSB-BP458161MVS
CSB-MP458161MVS
CSB-EP458161MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S9 (rpsI) CSB-YP458162MVS
CSB-EP458162MVS
CSB-BP458162MVS
CSB-MP458162MVS
CSB-EP458162MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Bifunctional protein GlmU (glmU) CSB-YP458163MVS
CSB-EP458163MVS
CSB-BP458163MVS
CSB-MP458163MVS
CSB-EP458163MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP458164MVS
CSB-EP458164MVS
CSB-BP458164MVS
CSB-MP458164MVS
CSB-EP458164MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP458165MVS
CSB-EP458165MVS
CSB-BP458165MVS
CSB-MP458165MVS
CSB-EP458165MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB 1, partial CSB-YP458166MVS
CSB-EP458166MVS
CSB-BP458166MVS
CSB-MP458166MVS
CSB-EP458166MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Probable DNA ligase (lig), partial CSB-YP458167MVS
CSB-EP458167MVS
CSB-BP458167MVS
CSB-MP458167MVS
CSB-EP458167MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Long-chain-fatty-acid--CoA ligase FadD17 (fadD17), partial CSB-YP458168MVS
CSB-EP458168MVS
CSB-BP458168MVS
CSB-MP458168MVS
CSB-EP458168MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP458169MVS
CSB-EP458169MVS
CSB-BP458169MVS
CSB-MP458169MVS
CSB-EP458169MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP458170MVS
CSB-EP458170MVS
CSB-BP458170MVS
CSB-MP458170MVS
CSB-EP458170MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Uncharacterized tRNA/rRNA methyltransferase MMAR_5079 (MMAR_5079) CSB-YP458171MVS
CSB-EP458171MVS
CSB-BP458171MVS
CSB-MP458171MVS
CSB-EP458171MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP458172MVS
CSB-EP458172MVS
CSB-BP458172MVS
CSB-MP458172MVS
CSB-EP458172MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP458173MVS
CSB-EP458173MVS
CSB-BP458173MVS
CSB-MP458173MVS
CSB-EP458173MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Ribosome maturation factor RimP (rimP) CSB-YP458174MVS
CSB-EP458174MVS
CSB-BP458174MVS
CSB-MP458174MVS
CSB-EP458174MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Dihydrodipicolinate reductase (dapB) CSB-YP458175MVS
CSB-EP458175MVS
CSB-BP458175MVS
CSB-MP458175MVS
CSB-EP458175MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Probable rRNA maturation factor (MMAR_3677) CSB-YP458176MVS
CSB-EP458176MVS
CSB-BP458176MVS
CSB-MP458176MVS
CSB-EP458176MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_0357 (MMAR_0357) CSB-YP458177MVS
CSB-EP458177MVS
CSB-BP458177MVS
CSB-MP458177MVS
CSB-EP458177MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Ribonuclease Z (rnz) CSB-YP458178MVS
CSB-EP458178MVS
CSB-BP458178MVS
CSB-MP458178MVS
CSB-EP458178MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L21 (rplU) CSB-YP458179MVS
CSB-EP458179MVS
CSB-BP458179MVS
CSB-MP458179MVS
CSB-EP458179MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP458180MVS
CSB-EP458180MVS
CSB-BP458180MVS
CSB-MP458180MVS
CSB-EP458180MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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