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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Amine oxidase [flavin-containing] B (MAOB), partial CSB-YP013399BO1
CSB-EP013399BO1
CSB-BP013399BO1
CSB-MP013399BO1
CSB-EP013399BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Orexin-A (HCRT) CSB-YP010230BO
CSB-EP010230BO
CSB-BP010230BO
CSB-MP010230BO
CSB-EP010230BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Claudin-12 (CLDN12), partial CSB-YP005493HU1
CSB-EP005493HU1
CSB-BP005493HU1
CSB-MP005493HU1
CSB-EP005493HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epididymal secretory protein E3-beta (EDDM3B) CSB-YP008053HU
CSB-EP008053HU
CSB-BP008053HU
CSB-MP008053HU
CSB-EP008053HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Niemann-Pick C1 protein (NPC1), partial CSB-YP015974PI
CSB-EP015974PI
CSB-BP015974PI
CSB-MP015974PI
CSB-EP015974PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Geranylgeranyl pyrophosphate synthase (GGPS1) CSB-YP009393BO
CSB-EP009393BO
CSB-BP009393BO
CSB-MP009393BO
CSB-EP009393BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (PIGP), partial CSB-YP017979HU1
CSB-EP017979HU1
CSB-BP017979HU1
CSB-MP017979HU1
CSB-EP017979HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PR domain zinc finger protein 15 (PRDM15), partial CSB-YP018644HU
CSB-EP018644HU
CSB-BP018644HU
CSB-MP018644HU
CSB-EP018644HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SCAN domain-containing protein 1 (SCAND1) CSB-YP020755HU
CSB-BP020755HU
CSB-MP020755HU
CSB-EP020755HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Gem-associated protein 4 (GEMIN4), partial CSB-YP009363HU
CSB-EP009363HU
CSB-BP009363HU
CSB-MP009363HU
CSB-EP009363HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Glycine receptor subunit alpha-1 (GLRA1), partial CSB-YP009516BO
CSB-EP009516BO
CSB-BP009516BO
CSB-MP009516BO
CSB-EP009516BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nicastrin (Ncstn), partial CSB-YP015600MO1
CSB-EP015600MO1
CSB-BP015600MO1
CSB-MP015600MO1
CSB-EP015600MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Poly (rC)-binding protein 4 (Pcbp4) CSB-YP017521MO
CSB-EP017521MO
CSB-BP017521MO
CSB-MP017521MO
CSB-EP017521MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse V-type proton ATPase subunit D (Atp6v1d) CSB-YP002401MO
CSB-EP002401MO
CSB-BP002401MO
CSB-MP002401MO
CSB-EP002401MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ficolin-2 (Fcn2) CSB-YP008551RA
CSB-EP008551RA
CSB-BP008551RA
CSB-MP008551RA
CSB-EP008551RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Gasdermin-D (GSDMD) CSB-YP009956HU
CSB-BP009956HU
CSB-MP009956HU
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Selenocysteine-specific elongation factor (EEFSEC) CSB-YP007436HU
CSB-EP007436HU
CSB-BP007436HU
CSB-MP007436HU
CSB-EP007436HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-actinin-4 (Actn4), partial CSB-YP001244MO
CSB-EP001244MO
CSB-BP001244MO
CSB-MP001244MO
CSB-EP001244MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium-binding protein 4 (CABP4) CSB-YP004390HU
CSB-EP004390HU
CSB-BP004390HU
CSB-MP004390HU
CSB-EP004390HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sestrin-1 (Sesn1) CSB-YP021095MO
CSB-EP021095MO
CSB-BP021095MO
CSB-MP021095MO
CSB-EP021095MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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