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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP458701DUG
CSB-EP458701DUG
CSB-BP458701DUG
CSB-MP458701DUG
CSB-EP458701DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Orotate phosphoribosyltransferase (pyrE) CSB-YP458702DUG
CSB-EP458702DUG
CSB-BP458702DUG
CSB-MP458702DUG
CSB-EP458702DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum V-type ATP synthase alpha chain (atpA), partial CSB-YP458703DUG
CSB-EP458703DUG
CSB-BP458703DUG
CSB-MP458703DUG
CSB-EP458703DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP458704DUG
CSB-EP458704DUG
CSB-BP458704DUG
CSB-MP458704DUG
CSB-EP458704DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Hydroxyethylthiazole kinase (thiM) CSB-YP458705DUG
CSB-EP458705DUG
CSB-BP458705DUG
CSB-MP458705DUG
CSB-EP458705DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP458706DUG
CSB-EP458706DUG
CSB-BP458706DUG
CSB-MP458706DUG
CSB-EP458706DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP458707DUG
CSB-EP458707DUG
CSB-BP458707DUG
CSB-MP458707DUG
CSB-EP458707DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP458708DUG
CSB-EP458708DUG
CSB-BP458708DUG
CSB-MP458708DUG
CSB-EP458708DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribonuclease P protein component (rnpA) CSB-YP458709DUG
CSB-EP458709DUG
CSB-BP458709DUG
CSB-MP458709DUG
CSB-EP458709DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP458710DUG
CSB-EP458710DUG
CSB-BP458710DUG
CSB-MP458710DUG
CSB-EP458710DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP458711DUG
CSB-EP458711DUG
CSB-BP458711DUG
CSB-MP458711DUG
CSB-EP458711DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein pyrR (pyrR) CSB-YP458712DUG
CSB-EP458712DUG
CSB-BP458712DUG
CSB-MP458712DUG
CSB-EP458712DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein MraZ (mraZ) CSB-YP458713DUG
CSB-EP458713DUG
CSB-BP458713DUG
CSB-MP458713DUG
CSB-EP458713DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Dihydrodipicolinate reductase (dapB) CSB-YP458714DUG
CSB-EP458714DUG
CSB-BP458714DUG
CSB-MP458714DUG
CSB-EP458714DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Bifunctional protein FolD (folD) CSB-YP458715SYZ
CSB-EP458715SYZ
CSB-BP458715SYZ
CSB-MP458715SYZ
CSB-EP458715SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Citrate lyase acyl carrier protein (citD) CSB-YP458716SYZ
CSB-EP458716SYZ
CSB-BP458716SYZ
CSB-MP458716SYZ
CSB-EP458716SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Dihydroorotase (pyrC) CSB-YP458717SYZ
CSB-EP458717SYZ
CSB-BP458717SYZ
CSB-MP458717SYZ
CSB-EP458717SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glucans biosynthesis protein C (mdoC), partial CSB-YP458718SYZ
CSB-EP458718SYZ
CSB-BP458718SYZ
CSB-MP458718SYZ
CSB-EP458718SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cell division inhibitor SulA (sulA) CSB-YP458719SYZ
CSB-EP458719SYZ
CSB-BP458719SYZ
CSB-MP458719SYZ
CSB-EP458719SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L28 (rpmB) CSB-YP458720SYZ
CSB-EP458720SYZ
CSB-BP458720SYZ
CSB-MP458720SYZ
CSB-EP458720SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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