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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Chromosomal replication initiator protein DnaA (dnaA) CSB-YP458641XBC
CSB-EP458641XBC
CSB-BP458641XBC
CSB-MP458641XBC
CSB-EP458641XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP458642XBC1
CSB-EP458642XBC1
CSB-BP458642XBC1
CSB-MP458642XBC1
CSB-EP458642XBC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP458643XBC
CSB-EP458643XBC
CSB-BP458643XBC
CSB-MP458643XBC
CSB-EP458643XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP458644XBC
CSB-EP458644XBC
CSB-BP458644XBC
CSB-MP458644XBC
CSB-EP458644XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Type III pantothenate kinase (coaX) CSB-YP458645BXU
CSB-EP458645BXU
CSB-BP458645BXU
CSB-MP458645BXU
CSB-EP458645BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP458646BXU
CSB-EP458646BXU
CSB-BP458646BXU
CSB-MP458646BXU
CSB-EP458646BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP458647BXU
CSB-EP458647BXU
CSB-BP458647BXU
CSB-MP458647BXU
CSB-EP458647BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Chaperone protein DnaJ (dnaJ) CSB-YP458648BXU
CSB-EP458648BXU
CSB-BP458648BXU
CSB-MP458648BXU
CSB-EP458648BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans HPr kinase/phosphorylase (hprK) CSB-YP458649BXU
CSB-EP458649BXU
CSB-BP458649BXU
CSB-MP458649BXU
CSB-EP458649BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Protein hfq (hfq) CSB-YP458650BXU
CSB-EP458650BXU
CSB-BP458650BXU
CSB-MP458650BXU
CSB-EP458650BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP458651BXU
CSB-EP458651BXU
CSB-BP458651BXU
CSB-MP458651BXU
CSB-EP458651BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L13 (rplM) CSB-YP458652BXU
CSB-EP458652BXU
CSB-BP458652BXU
CSB-MP458652BXU
CSB-EP458652BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L21 (rplU) CSB-YP458653BXU
CSB-EP458653BXU
CSB-BP458653BXU
CSB-MP458653BXU
CSB-EP458653BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP458654BXU
CSB-EP458654BXU
CSB-BP458654BXU
CSB-MP458654BXU
CSB-EP458654BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP458655BXU
CSB-EP458655BXU
CSB-BP458655BXU
CSB-MP458655BXU
CSB-EP458655BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Polyphosphate kinase (ppk), partial CSB-YP458656BXU
CSB-EP458656BXU
CSB-BP458656BXU
CSB-MP458656BXU
CSB-EP458656BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 10 kDa chaperonin (groS) CSB-YP458657BXU
CSB-EP458657BXU
CSB-BP458657BXU
CSB-MP458657BXU
CSB-EP458657BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Aspartate carbamoyltransferase (pyrB) CSB-YP458658BXU
CSB-EP458658BXU
CSB-BP458658BXU
CSB-MP458658BXU
CSB-EP458658BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Pantothenate synthetase (panC) CSB-YP458659BXU
CSB-EP458659BXU
CSB-BP458659BXU
CSB-MP458659BXU
CSB-EP458659BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Cell division topological specificity factor (minE) CSB-YP458660BXU
CSB-EP458660BXU
CSB-BP458660BXU
CSB-MP458660BXU
CSB-EP458660BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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