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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kocuria rhizophila Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP460101KBO
CSB-EP460101KBO
CSB-BP460101KBO
CSB-MP460101KBO
CSB-EP460101KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Urease accessory protein UreF (ureF) CSB-YP460102KBO
CSB-EP460102KBO
CSB-BP460102KBO
CSB-MP460102KBO
CSB-EP460102KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Mycothiol acetyltransferase (mshD) CSB-YP460103KBO
CSB-EP460103KBO
CSB-BP460103KBO
CSB-MP460103KBO
CSB-EP460103KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila UPF0678 fatty acid-binding protein-like protein KRH_05750 (KRH_05750) CSB-YP460104KBO
CSB-EP460104KBO
CSB-BP460104KBO
CSB-MP460104KBO
CSB-EP460104KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP460105KBO
CSB-EP460105KBO
CSB-BP460105KBO
CSB-MP460105KBO
CSB-EP460105KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L2 (rplB) CSB-YP460106KBO
CSB-EP460106KBO
CSB-BP460106KBO
CSB-MP460106KBO
CSB-EP460106KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L30 (rpmD) CSB-YP460107KBO
CSB-EP460107KBO
CSB-BP460107KBO
CSB-MP460107KBO
CSB-EP460107KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L13 (rplM) CSB-YP460108KBO
CSB-EP460108KBO
CSB-BP460108KBO
CSB-MP460108KBO
CSB-EP460108KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L9 (rplI) CSB-YP460109KBO
CSB-EP460109KBO
CSB-BP460109KBO
CSB-MP460109KBO
CSB-EP460109KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Protein MraZ (mraZ) CSB-YP460110KBO
CSB-EP460110KBO
CSB-BP460110KBO
CSB-MP460110KBO
CSB-EP460110KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Acetate kinase (ackA) CSB-YP460111KBO
CSB-EP460111KBO
CSB-BP460111KBO
CSB-MP460111KBO
CSB-EP460111KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP460112KBO
CSB-EP460112KBO
CSB-BP460112KBO
CSB-MP460112KBO
CSB-EP460112KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP460113KBO
CSB-EP460113KBO
CSB-BP460113KBO
CSB-MP460113KBO
CSB-EP460113KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP460114KBO
CSB-EP460114KBO
CSB-BP460114KBO
CSB-MP460114KBO
CSB-EP460114KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Spindle and kinetochore-associated protein 3 (Ska3) CSB-YP460115RA
CSB-EP460115RA
CSB-BP460115RA
CSB-MP460115RA
CSB-EP460115RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L24 (rplX) CSB-YP460116MVS
CSB-EP460116MVS
CSB-BP460116MVS
CSB-MP460116MVS
CSB-EP460116MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L15 (rplO) CSB-YP460117MVS
CSB-EP460117MVS
CSB-BP460117MVS
CSB-MP460117MVS
CSB-EP460117MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L36 (rpmJ) CSB-YP460118MVS
CSB-EP460118MVS
CSB-BP460118MVS
CSB-MP460118MVS
CSB-EP460118MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 10 kDa chaperonin (groS) CSB-YP460119MVS
CSB-EP460119MVS
CSB-BP460119MVS
CSB-MP460119MVS
CSB-EP460119MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Peptidyl-tRNA hydrolase (pth) CSB-YP460120MVS
CSB-EP460120MVS
CSB-BP460120MVS
CSB-MP460120MVS
CSB-EP460120MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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