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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella abortus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP460501BWR1
CSB-EP460501BWR1
CSB-BP460501BWR1
CSB-MP460501BWR1
CSB-EP460501BWR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP460502BWR
CSB-EP460502BWR
CSB-BP460502BWR
CSB-MP460502BWR
CSB-EP460502BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP460503BWR
CSB-EP460503BWR
CSB-BP460503BWR
CSB-MP460503BWR
CSB-EP460503BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Threonine--tRNA ligase (thrS), partial CSB-YP460504BWR
CSB-EP460504BWR
CSB-BP460504BWR
CSB-MP460504BWR
CSB-EP460504BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Phosphopantetheine adenylyltransferase (coaD) CSB-YP460505BWR
CSB-EP460505BWR
CSB-BP460505BWR
CSB-MP460505BWR
CSB-EP460505BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Anthranilate phosphoribosyltransferase (trpD) CSB-YP460506BWR
CSB-EP460506BWR
CSB-BP460506BWR
CSB-MP460506BWR
CSB-EP460506BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus LexA repressor (lexA) CSB-YP460507BWR
CSB-EP460507BWR
CSB-BP460507BWR
CSB-MP460507BWR
CSB-EP460507BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S13 (rpsM) CSB-YP460508BWR
CSB-EP460508BWR
CSB-BP460508BWR
CSB-MP460508BWR
CSB-EP460508BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S5 (rpsE) CSB-YP460509BWR
CSB-EP460509BWR
CSB-BP460509BWR
CSB-MP460509BWR
CSB-EP460509BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S8 (rpsH) CSB-YP460510BWR
CSB-EP460510BWR
CSB-BP460510BWR
CSB-MP460510BWR
CSB-EP460510BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L23 (rplW) CSB-YP460511BWR
CSB-EP460511BWR
CSB-BP460511BWR
CSB-MP460511BWR
CSB-EP460511BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Probable potassium transport system protein kup (kup), partial CSB-YP460512BWR
CSB-EP460512BWR
CSB-BP460512BWR
CSB-MP460512BWR
CSB-EP460512BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP460513BWR
CSB-EP460513BWR
CSB-BP460513BWR
CSB-MP460513BWR
CSB-EP460513BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Transcription elongation factor GreA (greA) CSB-YP460514BWR
CSB-EP460514BWR
CSB-BP460514BWR
CSB-MP460514BWR
CSB-EP460514BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L36 (rpmJ) CSB-YP460515BWR
CSB-EP460515BWR
CSB-BP460515BWR
CSB-MP460515BWR
CSB-EP460515BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus RNA pyrophosphohydrolase (rppH) CSB-YP460516BWR
CSB-EP460516BWR
CSB-BP460516BWR
CSB-MP460516BWR
CSB-EP460516BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP460517BWR
CSB-EP460517BWR
CSB-BP460517BWR
CSB-MP460517BWR
CSB-EP460517BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Integration host factor subunit beta (ihfB) CSB-YP460518BWR
CSB-EP460518BWR
CSB-BP460518BWR
CSB-MP460518BWR
CSB-EP460518BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Shikimate kinase (aroK) CSB-YP460519BWR
CSB-EP460519BWR
CSB-BP460519BWR
CSB-MP460519BWR
CSB-EP460519BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Dihydroorotate dehydrogenase (quinone) CSB-YP460520BWR
CSB-EP460520BWR
CSB-BP460520BWR
CSB-MP460520BWR
CSB-EP460520BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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