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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cupriavidus taiwanensis 50S ribosomal protein L27 (rpmA) CSB-YP459461DZS
CSB-EP459461DZS
CSB-BP459461DZS
CSB-MP459461DZS
CSB-EP459461DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP459462DZS
CSB-EP459462DZS
CSB-BP459462DZS
CSB-MP459462DZS
CSB-EP459462DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S1-A (RPS1A) CSB-YP459463SVP
CSB-EP459463SVP
CSB-BP459463SVP
CSB-MP459463SVP
CSB-EP459463SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Ribosome biogenesis protein WDR12 homolog (TRIADDRAFT_55049) CSB-YP459464TQD
CSB-EP459464TQD
CSB-BP459464TQD
CSB-MP459464TQD
CSB-EP459464TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Glutamyl-tRNA (Gln) amidotransferase subunit C, mitochondrial, partial CSB-YP459465TQD
CSB-EP459465TQD
CSB-BP459465TQD
CSB-MP459465TQD
CSB-EP459465TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP459466RNV
CSB-EP459466RNV
CSB-BP459466RNV
CSB-MP459466RNV
CSB-EP459466RNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 3 CSB-YP459467ROI
CSB-EP459467ROI
CSB-BP459467ROI
CSB-MP459467ROI
CSB-EP459467ROI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP459468ROL
CSB-EP459468ROL
CSB-BP459468ROL
CSB-MP459468ROL
CSB-EP459468ROL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nosema bombycis Spore wall protein 25 (SWP25) CSB-YP459469NHN
CSB-EP459469NHN
CSB-BP459469NHN
CSB-MP459469NHN
CSB-EP459469NHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP459470BVS1
CSB-EP459470BVS1
CSB-BP459470BVS1
CSB-MP459470BVS1
CSB-EP459470BVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP459471BVS
CSB-EP459471BVS
CSB-BP459471BVS
CSB-MP459471BVS
CSB-EP459471BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP459472BVS
CSB-EP459472BVS
CSB-BP459472BVS
CSB-MP459472BVS
CSB-EP459472BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP459473BVS
CSB-EP459473BVS
CSB-BP459473BVS
CSB-MP459473BVS
CSB-EP459473BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP459474BVS1
CSB-EP459474BVS1
CSB-BP459474BVS1
CSB-MP459474BVS1
CSB-EP459474BVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ourmia melon virus Putative movement protein CSB-YP459475OFU
CSB-EP459475OFU
CSB-BP459475OFU
CSB-MP459475OFU
CSB-EP459475OFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana dybowskii Temporin-CDYb CSB-YP459476RJK
CSB-EP459476RJK
CSB-BP459476RJK
CSB-MP459476RJK
CSB-EP459476RJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Tryptophan synthase beta chain (trpB) CSB-YP459477LMJ
CSB-EP459477LMJ
CSB-BP459477LMJ
CSB-MP459477LMJ
CSB-EP459477LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0397 protein LCABL_04350 (LCABL_04350), partial CSB-YP459478LMJ1
CSB-EP459478LMJ1
CSB-BP459478LMJ1
CSB-MP459478LMJ1
CSB-EP459478LMJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei N-acetyldiaminopimelate deacetylase (LCABL_00980) CSB-YP459479LMJ
CSB-EP459479LMJ
CSB-BP459479LMJ
CSB-MP459479LMJ
CSB-EP459479LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP459480LMJ
CSB-EP459480LMJ
CSB-BP459480LMJ
CSB-MP459480LMJ
CSB-EP459480LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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