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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorobaculum parvum Type III pantothenate kinase (coaX) CSB-YP459381DSQ
CSB-EP459381DSQ
CSB-BP459381DSQ
CSB-MP459381DSQ
CSB-EP459381DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Lysine--tRNA ligase (lysS), partial CSB-YP459382DSQ
CSB-EP459382DSQ
CSB-BP459382DSQ
CSB-MP459382DSQ
CSB-EP459382DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum ATP synthase subunit a 1 (atpB1), partial CSB-YP459383DSQ1
CSB-EP459383DSQ1
CSB-BP459383DSQ1
CSB-MP459383DSQ1
CSB-EP459383DSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Cobalamin synthase (cobS), partial CSB-YP459384DSQ1
CSB-EP459384DSQ1
CSB-BP459384DSQ1
CSB-MP459384DSQ1
CSB-EP459384DSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP459385DSQ
CSB-EP459385DSQ
CSB-BP459385DSQ
CSB-MP459385DSQ
CSB-EP459385DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 7-cyano-7-deazaguanine synthase (queC) CSB-YP459386DSQ
CSB-EP459386DSQ
CSB-BP459386DSQ
CSB-MP459386DSQ
CSB-EP459386DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Chaperone protein DnaJ (dnaJ) CSB-YP459387DSQ
CSB-EP459387DSQ
CSB-BP459387DSQ
CSB-MP459387DSQ
CSB-EP459387DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Nitrogenase iron protein (nifH) CSB-YP459388DSQ
CSB-EP459388DSQ
CSB-BP459388DSQ
CSB-MP459388DSQ
CSB-EP459388DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Undecaprenyl-diphosphatase (uppP), partial CSB-YP459389DSQ1
CSB-EP459389DSQ1
CSB-BP459389DSQ1
CSB-MP459389DSQ1
CSB-EP459389DSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S15 (rpsO) CSB-YP459390DSQ
CSB-EP459390DSQ
CSB-BP459390DSQ
CSB-MP459390DSQ
CSB-EP459390DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Elongation factor P (efp) CSB-YP459391DSQ
CSB-EP459391DSQ
CSB-BP459391DSQ
CSB-MP459391DSQ
CSB-EP459391DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S21 (rpsU) CSB-YP459392DSQ
CSB-EP459392DSQ
CSB-BP459392DSQ
CSB-MP459392DSQ
CSB-EP459392DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP459393DSQ
CSB-EP459393DSQ
CSB-BP459393DSQ
CSB-MP459393DSQ
CSB-EP459393DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S12 (rpsL) CSB-YP459394DSQ
CSB-EP459394DSQ
CSB-BP459394DSQ
CSB-MP459394DSQ
CSB-EP459394DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L24 (rplX) CSB-YP459395DSQ
CSB-EP459395DSQ
CSB-BP459395DSQ
CSB-MP459395DSQ
CSB-EP459395DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L2 (rplB) CSB-YP459396DSQ
CSB-EP459396DSQ
CSB-BP459396DSQ
CSB-MP459396DSQ
CSB-EP459396DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L36 (rpmJ) CSB-YP459397DSQ
CSB-EP459397DSQ
CSB-BP459397DSQ
CSB-MP459397DSQ
CSB-EP459397DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP459398DSQ
CSB-EP459398DSQ
CSB-BP459398DSQ
CSB-MP459398DSQ
CSB-EP459398DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L35 (rpmI) CSB-YP459399DSQ
CSB-EP459399DSQ
CSB-BP459399DSQ
CSB-MP459399DSQ
CSB-EP459399DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Leucine--tRNA ligase (leuS), partial CSB-YP459400DSY
CSB-EP459400DSY
CSB-BP459400DSY
CSB-MP459400DSY
CSB-EP459400DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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