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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pneumoniae Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP460261FMZ
CSB-EP460261FMZ
CSB-BP460261FMZ
CSB-MP460261FMZ
CSB-EP460261FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribonuclease HII (rnhB) CSB-YP460262FMZ
CSB-EP460262FMZ
CSB-BP460262FMZ
CSB-MP460262FMZ
CSB-EP460262FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Enolase (eno) CSB-YP460263FMZ
CSB-EP460263FMZ
CSB-BP460263FMZ
CSB-MP460263FMZ
CSB-EP460263FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP460264FMZ
CSB-EP460264FMZ
CSB-BP460264FMZ
CSB-MP460264FMZ
CSB-EP460264FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 3-dehydroquinate synthase (aroB) CSB-YP460265FMZ
CSB-EP460265FMZ
CSB-BP460265FMZ
CSB-MP460265FMZ
CSB-EP460265FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP460266FMZ
CSB-EP460266FMZ
CSB-BP460266FMZ
CSB-MP460266FMZ
CSB-EP460266FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA (Ile)-lysidine synthase CSB-YP460267FMZ
CSB-EP460267FMZ
CSB-BP460267FMZ
CSB-MP460267FMZ
CSB-EP460267FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae CCA-adding enzyme (cca) CSB-YP460268FMZ
CSB-EP460268FMZ
CSB-BP460268FMZ
CSB-MP460268FMZ
CSB-EP460268FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP460269FMZ
CSB-EP460269FMZ
CSB-BP460269FMZ
CSB-MP460269FMZ
CSB-EP460269FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 30S ribosomal protein S4 (rpsD) CSB-YP460270FMZ
CSB-EP460270FMZ
CSB-BP460270FMZ
CSB-MP460270FMZ
CSB-EP460270FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP460271FMZ
CSB-EP460271FMZ
CSB-BP460271FMZ
CSB-MP460271FMZ
CSB-EP460271FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Putative Holliday junction resolvase (SPCG_0206) CSB-YP460272FMZ
CSB-EP460272FMZ
CSB-BP460272FMZ
CSB-MP460272FMZ
CSB-EP460272FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L5 (rplE) CSB-YP460273FMZ
CSB-EP460273FMZ
CSB-BP460273FMZ
CSB-MP460273FMZ
CSB-EP460273FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L30 (rpmD) CSB-YP460274FMZ
CSB-EP460274FMZ
CSB-BP460274FMZ
CSB-MP460274FMZ
CSB-EP460274FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L17 (rplQ) CSB-YP460275FMZ
CSB-EP460275FMZ
CSB-BP460275FMZ
CSB-MP460275FMZ
CSB-EP460275FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Proline--tRNA ligase (proS), partial CSB-YP460276FMZ
CSB-EP460276FMZ
CSB-BP460276FMZ
CSB-MP460276FMZ
CSB-EP460276FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Anthranilate phosphoribosyltransferase (trpD) CSB-YP460277FMZ
CSB-EP460277FMZ
CSB-BP460277FMZ
CSB-MP460277FMZ
CSB-EP460277FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Dihydrodipicolinate synthase (dapA) CSB-YP460278NHQ
CSB-EP460278NHQ
CSB-BP460278NHQ
CSB-MP460278NHQ
CSB-EP460278NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L36 (rpmJ) CSB-YP460279NHQ
CSB-EP460279NHQ
CSB-BP460279NHQ
CSB-MP460279NHQ
CSB-EP460279NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L5 (rplE) CSB-YP460280NHQ
CSB-EP460280NHQ
CSB-BP460280NHQ
CSB-MP460280NHQ
CSB-EP460280NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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