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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Chlorobium limicola 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP461061DSR
CSB-EP461061DSR
CSB-BP461061DSR
CSB-MP461061DSR
CSB-EP461061DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP461062DSR
CSB-EP461062DSR
CSB-BP461062DSR
CSB-MP461062DSR
CSB-EP461062DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP461063DSR
CSB-EP461063DSR
CSB-BP461063DSR
CSB-MP461063DSR
CSB-EP461063DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L17 (rplQ) CSB-YP461064DSR
CSB-EP461064DSR
CSB-BP461064DSR
CSB-MP461064DSR
CSB-EP461064DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S5 (rpsE) CSB-YP461065DSR
CSB-EP461065DSR
CSB-BP461065DSR
CSB-MP461065DSR
CSB-EP461065DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L16 (rplP) CSB-YP461066DSR
CSB-EP461066DSR
CSB-BP461066DSR
CSB-MP461066DSR
CSB-EP461066DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP461067DSR
CSB-EP461067DSR
CSB-BP461067DSR
CSB-MP461067DSR
CSB-EP461067DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola HPr kinase/phosphorylase (hprK) CSB-YP461068DSR
CSB-EP461068DSR
CSB-BP461068DSR
CSB-MP461068DSR
CSB-EP461068DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Ribonuclease H (rnhA) CSB-YP461069DSR
CSB-EP461069DSR
CSB-BP461069DSR
CSB-MP461069DSR
CSB-EP461069DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S10 (rpsJ) CSB-YP461070DSR
CSB-EP461070DSR
CSB-BP461070DSR
CSB-MP461070DSR
CSB-EP461070DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Chaperone protein DnaK (dnaK), partial CSB-YP461071DSR
CSB-EP461071DSR
CSB-BP461071DSR
CSB-MP461071DSR
CSB-EP461071DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP461072DSR
CSB-EP461072DSR
CSB-BP461072DSR
CSB-MP461072DSR
CSB-EP461072DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP461073DSR
CSB-EP461073DSR
CSB-BP461073DSR
CSB-MP461073DSR
CSB-EP461073DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP461074DSR
CSB-EP461074DSR
CSB-BP461074DSR
CSB-MP461074DSR
CSB-EP461074DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP461075DSR
CSB-EP461075DSR
CSB-BP461075DSR
CSB-MP461075DSR
CSB-EP461075DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Protein translocase subunit SecA (secA), partial CSB-YP461076DSS
CSB-EP461076DSS
CSB-BP461076DSS
CSB-MP461076DSS
CSB-EP461076DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP461077DSS
CSB-EP461077DSS
CSB-BP461077DSS
CSB-MP461077DSS
CSB-EP461077DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Cobyric acid synthase (cobQ) CSB-YP461078DSS
CSB-EP461078DSS
CSB-BP461078DSS
CSB-MP461078DSS
CSB-EP461078DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S6 (rpsF) CSB-YP461079DSS
CSB-EP461079DSS
CSB-BP461079DSS
CSB-MP461079DSS
CSB-EP461079DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Threonine--tRNA ligase (thrS), partial CSB-YP461080DSS
CSB-EP461080DSS
CSB-BP461080DSS
CSB-MP461080DSS
CSB-EP461080DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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