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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Exiguobacterium sibiricum Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP459881EPT
CSB-EP459881EPT
CSB-BP459881EPT
CSB-MP459881EPT
CSB-EP459881EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Putative sporulation transcription regulator WhiA (whiA) CSB-YP459882EPT
CSB-EP459882EPT
CSB-BP459882EPT
CSB-MP459882EPT
CSB-EP459882EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP459883EPT
CSB-EP459883EPT
CSB-BP459883EPT
CSB-MP459883EPT
CSB-EP459883EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Anthranilate phosphoribosyltransferase (trpD) CSB-YP459884EPT
CSB-EP459884EPT
CSB-BP459884EPT
CSB-MP459884EPT
CSB-EP459884EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Phosphopentomutase (deoB) CSB-YP459885EPT
CSB-EP459885EPT
CSB-BP459885EPT
CSB-MP459885EPT
CSB-EP459885EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Protein hfq (hfq) CSB-YP459886EPT
CSB-EP459886EPT
CSB-BP459886EPT
CSB-MP459886EPT
CSB-EP459886EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Phosphoglucosamine mutase (glmM) CSB-YP459887BXP
CSB-EP459887BXP
CSB-BP459887BXP
CSB-MP459887BXP
CSB-EP459887BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Peptidyl-tRNA hydrolase (pth) CSB-YP459888BXP
CSB-EP459888BXP
CSB-BP459888BXP
CSB-MP459888BXP
CSB-EP459888BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Octanoyltransferase (lipB) CSB-YP459889BXP
CSB-EP459889BXP
CSB-BP459889BXP
CSB-MP459889BXP
CSB-EP459889BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Chorismate synthase (aroC) CSB-YP459890BXP
CSB-EP459890BXP
CSB-BP459890BXP
CSB-MP459890BXP
CSB-EP459890BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Ribose-5-phosphate isomerase A (rpiA) CSB-YP459891BXP
CSB-EP459891BXP
CSB-BP459891BXP
CSB-MP459891BXP
CSB-EP459891BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP459892BXP
CSB-EP459892BXP
CSB-BP459892BXP
CSB-MP459892BXP
CSB-EP459892BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Putative membrane protein insertion efficiency factor (BamMC406_3100) CSB-YP459893BXP
CSB-EP459893BXP
CSB-BP459893BXP
CSB-MP459893BXP
CSB-EP459893BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria GTPase Der (der) CSB-YP459894BXP
CSB-EP459894BXP
CSB-BP459894BXP
CSB-MP459894BXP
CSB-EP459894BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L7/L12 (rplL) CSB-YP459895BXP
CSB-EP459895BXP
CSB-BP459895BXP
CSB-MP459895BXP
CSB-EP459895BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L4 (rplD) CSB-YP459896BXP
CSB-EP459896BXP
CSB-BP459896BXP
CSB-MP459896BXP
CSB-EP459896BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP459897BXP
CSB-EP459897BXP
CSB-BP459897BXP
CSB-MP459897BXP
CSB-EP459897BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S6 (rpsF) CSB-YP459898BXP
CSB-EP459898BXP
CSB-BP459898BXP
CSB-MP459898BXP
CSB-EP459898BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S14 (rpsN) CSB-YP459899BXP
CSB-EP459899BXP
CSB-BP459899BXP
CSB-MP459899BXP
CSB-EP459899BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S13 (rpsM) CSB-YP459900BXP
CSB-EP459900BXP
CSB-BP459900BXP
CSB-MP459900BXP
CSB-EP459900BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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