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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus fermentum 30S ribosomal protein S2 (rpsB) CSB-YP460081LMO
CSB-EP460081LMO
CSB-BP460081LMO
CSB-MP460081LMO
CSB-EP460081LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP460082LMO
CSB-EP460082LMO
CSB-BP460082LMO
CSB-MP460082LMO
CSB-EP460082LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Hydroxyethylthiazole kinase (thiM) CSB-YP460083LMO
CSB-EP460083LMO
CSB-BP460083LMO
CSB-MP460083LMO
CSB-EP460083LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum UPF0210 protein LAF_0976 (LAF_0976) CSB-YP460084LMO
CSB-EP460084LMO
CSB-BP460084LMO
CSB-MP460084LMO
CSB-EP460084LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum tRNA (guanine-N (1)-)-methyltransferase CSB-YP460085LMO
CSB-EP460085LMO
CSB-BP460085LMO
CSB-MP460085LMO
CSB-EP460085LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L20 (rplT) CSB-YP460086LMO
CSB-EP460086LMO
CSB-BP460086LMO
CSB-MP460086LMO
CSB-EP460086LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum UPF0342 protein LAF_1331 (LAF_1331) CSB-YP460087LMO
CSB-EP460087LMO
CSB-BP460087LMO
CSB-MP460087LMO
CSB-EP460087LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Xanthine phosphoribosyltransferase (xpt) CSB-YP460088LMO
CSB-EP460088LMO
CSB-BP460088LMO
CSB-MP460088LMO
CSB-EP460088LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 30S ribosomal protein S13 (rpsM) CSB-YP460089LMO
CSB-EP460089LMO
CSB-BP460089LMO
CSB-MP460089LMO
CSB-EP460089LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L15 (rplO) CSB-YP460090LMO
CSB-EP460090LMO
CSB-BP460090LMO
CSB-MP460090LMO
CSB-EP460090LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L4 (rplD) CSB-YP460091LMO
CSB-EP460091LMO
CSB-BP460091LMO
CSB-MP460091LMO
CSB-EP460091LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP460092LMO
CSB-EP460092LMO
CSB-BP460092LMO
CSB-MP460092LMO
CSB-EP460092LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum UPF0145 protein LAF_1635 (LAF_1635) CSB-YP460093LMO
CSB-EP460093LMO
CSB-BP460093LMO
CSB-MP460093LMO
CSB-EP460093LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Large-conductance mechanosensitive channel (mscL), partial CSB-YP460094LMO1
CSB-EP460094LMO1
CSB-BP460094LMO1
CSB-MP460094LMO1
CSB-EP460094LMO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP460095LMO
CSB-EP460095LMO
CSB-BP460095LMO
CSB-MP460095LMO
CSB-EP460095LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP460096LMO
CSB-EP460096LMO
CSB-BP460096LMO
CSB-MP460096LMO
CSB-EP460096LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP460097KBO
CSB-EP460097KBO
CSB-BP460097KBO
CSB-MP460097KBO
CSB-EP460097KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L19 (rplS) CSB-YP460098KBO
CSB-EP460098KBO
CSB-BP460098KBO
CSB-MP460098KBO
CSB-EP460098KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S14 (rpsN) CSB-YP460099KBO
CSB-EP460099KBO
CSB-BP460099KBO
CSB-MP460099KBO
CSB-EP460099KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 50S ribosomal protein L21 (rplU) CSB-YP460100KBO
CSB-EP460100KBO
CSB-BP460100KBO
CSB-MP460100KBO
CSB-EP460100KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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