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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S27e (rps27e) CSB-YP459841TNE
CSB-EP459841TNE
CSB-BP459841TNE
CSB-MP459841TNE
CSB-EP459841TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Leucine--tRNA ligase (leuS), partial CSB-YP459842TNE
CSB-EP459842TNE
CSB-BP459842TNE
CSB-MP459842TNE
CSB-EP459842TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Uncharacterized DAPA-like lyase Tneu_1812 (dapAL) CSB-YP459843TNE
CSB-EP459843TNE
CSB-BP459843TNE
CSB-MP459843TNE
CSB-EP459843TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP459844TNE
CSB-EP459844TNE
CSB-BP459844TNE
CSB-MP459844TNE
CSB-EP459844TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP459845TNE
CSB-EP459845TNE
CSB-BP459845TNE
CSB-MP459845TNE
CSB-EP459845TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP459846TNE
CSB-EP459846TNE
CSB-BP459846TNE
CSB-MP459846TNE
CSB-EP459846TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Probable translation initiation factor IF-2 (infB), partial CSB-YP459847TNE
CSB-EP459847TNE
CSB-BP459847TNE
CSB-MP459847TNE
CSB-EP459847TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S19P (rps19p) CSB-YP459848TNE
CSB-EP459848TNE
CSB-BP459848TNE
CSB-MP459848TNE
CSB-EP459848TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 3-dehydroquinate synthase (aroB) CSB-YP459849TNE
CSB-EP459849TNE
CSB-BP459849TNE
CSB-MP459849TNE
CSB-EP459849TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L10e (rpl10e) CSB-YP459850TNE
CSB-EP459850TNE
CSB-BP459850TNE
CSB-MP459850TNE
CSB-EP459850TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Putative ribose 1,5-bisphosphate isomerase (Tneu_1052) CSB-YP459851TNE
CSB-EP459851TNE
CSB-BP459851TNE
CSB-MP459851TNE
CSB-EP459851TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ribose-5-phosphate isomerase A (rpiA) CSB-YP459852EPT
CSB-EP459852EPT
CSB-BP459852EPT
CSB-MP459852EPT
CSB-EP459852EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 60 kDa chaperonin (groL), partial CSB-YP459853EPT
CSB-EP459853EPT
CSB-BP459853EPT
CSB-MP459853EPT
CSB-EP459853EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 7-cyano-7-deazaguanine synthase (queC) CSB-YP459854EPT
CSB-EP459854EPT
CSB-BP459854EPT
CSB-MP459854EPT
CSB-EP459854EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L9 (rplI) CSB-YP459855EPT
CSB-EP459855EPT
CSB-BP459855EPT
CSB-MP459855EPT
CSB-EP459855EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum DNA replication and repair protein recF (recF) CSB-YP459856EPT
CSB-EP459856EPT
CSB-BP459856EPT
CSB-MP459856EPT
CSB-EP459856EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Putative septation protein spoVG (spoVG) CSB-YP459857EPT
CSB-EP459857EPT
CSB-BP459857EPT
CSB-MP459857EPT
CSB-EP459857EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L11 (rplK) CSB-YP459858EPT
CSB-EP459858EPT
CSB-BP459858EPT
CSB-MP459858EPT
CSB-EP459858EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L4 (rplD) CSB-YP459859EPT
CSB-EP459859EPT
CSB-BP459859EPT
CSB-MP459859EPT
CSB-EP459859EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L29 (rpmC) CSB-YP459860EPT
CSB-EP459860EPT
CSB-BP459860EPT
CSB-MP459860EPT
CSB-EP459860EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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