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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Opitutus terrae Cysteine--tRNA ligase (cysS) CSB-YP459961OEN
CSB-EP459961OEN
CSB-BP459961OEN
CSB-MP459961OEN
CSB-EP459961OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP459962OEN
CSB-EP459962OEN
CSB-BP459962OEN
CSB-MP459962OEN
CSB-EP459962OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP459963OEN
CSB-EP459963OEN
CSB-BP459963OEN
CSB-MP459963OEN
CSB-EP459963OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP459964OEN
CSB-EP459964OEN
CSB-BP459964OEN
CSB-MP459964OEN
CSB-EP459964OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 50S ribosomal protein L32 (rpmF) CSB-YP459965OEN
CSB-EP459965OEN
CSB-BP459965OEN
CSB-MP459965OEN
CSB-EP459965OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae tRNA modification GTPase MnmE (mnmE) CSB-YP459966OEN
CSB-EP459966OEN
CSB-BP459966OEN
CSB-MP459966OEN
CSB-EP459966OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae UPF0502 protein Oter_3715 (Oter_3715) CSB-YP459967OEN
CSB-EP459967OEN
CSB-BP459967OEN
CSB-MP459967OEN
CSB-EP459967OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae GTPase obg (obg) CSB-YP459968OEN
CSB-EP459968OEN
CSB-BP459968OEN
CSB-MP459968OEN
CSB-EP459968OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Probable DNA ligase (lig), partial CSB-YP459969OEN
CSB-EP459969OEN
CSB-BP459969OEN
CSB-MP459969OEN
CSB-EP459969OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP459970NFO
CSB-EP459970NFO
CSB-BP459970NFO
CSB-MP459970NFO
CSB-EP459970NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0317 protein Nther_1142 (Nther_1142) CSB-YP459971NFO
CSB-EP459971NFO
CSB-BP459971NFO
CSB-MP459971NFO
CSB-EP459971NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP459972NFO
CSB-EP459972NFO
CSB-BP459972NFO
CSB-MP459972NFO
CSB-EP459972NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP459973NFO
CSB-EP459973NFO
CSB-BP459973NFO
CSB-MP459973NFO
CSB-EP459973NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus tRNA (guanine-N (1)-)-methyltransferase CSB-YP459974NFO
CSB-EP459974NFO
CSB-BP459974NFO
CSB-MP459974NFO
CSB-EP459974NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP459975NFO
CSB-EP459975NFO
CSB-BP459975NFO
CSB-MP459975NFO
CSB-EP459975NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Tyrosine recombinase XerC (xerC) CSB-YP459976NFO
CSB-EP459976NFO
CSB-BP459976NFO
CSB-MP459976NFO
CSB-EP459976NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Ribosome maturation factor RimP (rimP) CSB-YP459977NFO
CSB-EP459977NFO
CSB-BP459977NFO
CSB-MP459977NFO
CSB-EP459977NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Putative competence-damage inducible protein (cinA) CSB-YP459978NFO
CSB-EP459978NFO
CSB-BP459978NFO
CSB-MP459978NFO
CSB-EP459978NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP459979NFO
CSB-EP459979NFO
CSB-BP459979NFO
CSB-MP459979NFO
CSB-EP459979NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S6 (rpsF) CSB-YP459980NFO
CSB-EP459980NFO
CSB-BP459980NFO
CSB-MP459980NFO
CSB-EP459980NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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