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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum Chorismate synthase (aroC) CSB-YP460141MVS
CSB-EP460141MVS
CSB-BP460141MVS
CSB-MP460141MVS
CSB-EP460141MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP460142MVS
CSB-EP460142MVS
CSB-BP460142MVS
CSB-MP460142MVS
CSB-EP460142MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Deoxycytidine triphosphate deaminase (dcd) CSB-YP460143MVS
CSB-EP460143MVS
CSB-BP460143MVS
CSB-MP460143MVS
CSB-EP460143MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UvrABC system protein C (uvrC), partial CSB-YP460144MVS
CSB-EP460144MVS
CSB-BP460144MVS
CSB-MP460144MVS
CSB-EP460144MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Thiamine-phosphate synthase (thiE) CSB-YP460145MVS
CSB-EP460145MVS
CSB-BP460145MVS
CSB-MP460145MVS
CSB-EP460145MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP460146MVS
CSB-EP460146MVS
CSB-BP460146MVS
CSB-MP460146MVS
CSB-EP460146MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L35 (rpmI) CSB-YP460147MVS
CSB-EP460147MVS
CSB-BP460147MVS
CSB-MP460147MVS
CSB-EP460147MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum o-succinylbenzoate synthase (menC) CSB-YP460148MVS
CSB-EP460148MVS
CSB-BP460148MVS
CSB-MP460148MVS
CSB-EP460148MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Protease HtpX homolog (htpX), partial CSB-YP460149MVS1
CSB-EP460149MVS1
CSB-BP460149MVS1
CSB-MP460149MVS1
CSB-EP460149MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (mshB) CSB-YP460150MVS
CSB-EP460150MVS
CSB-BP460150MVS
CSB-MP460150MVS
CSB-EP460150MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S7 (rpsG) CSB-YP460151MVS
CSB-EP460151MVS
CSB-BP460151MVS
CSB-MP460151MVS
CSB-EP460151MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L3 (rplC) CSB-YP460152MVS
CSB-EP460152MVS
CSB-BP460152MVS
CSB-MP460152MVS
CSB-EP460152MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L22 (rplV) CSB-YP460153MVS
CSB-EP460153MVS
CSB-BP460153MVS
CSB-MP460153MVS
CSB-EP460153MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protein-export protein secB (secB) CSB-YP460154AWO
CSB-EP460154AWO
CSB-BP460154AWO
CSB-MP460154AWO
CSB-EP460154AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L20 (rplT) CSB-YP460155AWO
CSB-EP460155AWO
CSB-BP460155AWO
CSB-MP460155AWO
CSB-EP460155AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP460156AWO1
CSB-EP460156AWO1
CSB-BP460156AWO1
CSB-MP460156AWO1
CSB-EP460156AWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 30S ribosomal protein S18 (rpsR) CSB-YP460157AWO
CSB-EP460157AWO
CSB-BP460157AWO
CSB-MP460157AWO
CSB-EP460157AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP460158AWO
CSB-EP460158AWO
CSB-BP460158AWO
CSB-MP460158AWO
CSB-EP460158AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP460159AWO
CSB-EP460159AWO
CSB-BP460159AWO
CSB-MP460159AWO
CSB-EP460159AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Multifunctional CCA protein (cca) CSB-YP460160AWO
CSB-EP460160AWO
CSB-BP460160AWO
CSB-MP460160AWO
CSB-EP460160AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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