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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L19 (rplS) CSB-YP460041FLX
CSB-EP460041FLX
CSB-BP460041FLX
CSB-MP460041FLX
CSB-EP460041FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii UTP--glucose-1-phosphate uridylyltransferase AglF (aglF) CSB-YP460042HTT
CSB-EP460042HTT
CSB-BP460042HTT
CSB-MP460042HTT
CSB-EP460042HTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S6 (rpsF) CSB-YP460043LMV
CSB-EP460043LMV
CSB-BP460043LMV
CSB-MP460043LMV
CSB-EP460043LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP460044LMV
CSB-EP460044LMV
CSB-BP460044LMV
CSB-MP460044LMV
CSB-EP460044LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L31 type B (rpmE2) CSB-YP460045LMV
CSB-EP460045LMV
CSB-BP460045LMV
CSB-MP460045LMV
CSB-EP460045LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri NH (3)-dependent NAD (+) synthetase CSB-YP460046LMV
CSB-EP460046LMV
CSB-BP460046LMV
CSB-MP460046LMV
CSB-EP460046LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Gamma-glutamyl phosphate reductase (proA) CSB-YP460047LMV
CSB-EP460047LMV
CSB-BP460047LMV
CSB-MP460047LMV
CSB-EP460047LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 60 kDa chaperonin (groL), partial CSB-YP460048LMV
CSB-EP460048LMV
CSB-BP460048LMV
CSB-MP460048LMV
CSB-EP460048LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Putative sporulation transcription regulator WhiA (whiA) CSB-YP460049LMV
CSB-EP460049LMV
CSB-BP460049LMV
CSB-MP460049LMV
CSB-EP460049LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri ATP synthase gamma chain (atpG) CSB-YP460050LMV
CSB-EP460050LMV
CSB-BP460050LMV
CSB-MP460050LMV
CSB-EP460050LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA mismatch repair protein MutS (mutS), partial CSB-YP460051LMV
CSB-EP460051LMV
CSB-BP460051LMV
CSB-MP460051LMV
CSB-EP460051LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP460052LMV
CSB-EP460052LMV
CSB-BP460052LMV
CSB-MP460052LMV
CSB-EP460052LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Glutamate racemase (murI) CSB-YP460053LMV
CSB-EP460053LMV
CSB-BP460053LMV
CSB-MP460053LMV
CSB-EP460053LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP460054LMV
CSB-EP460054LMV
CSB-BP460054LMV
CSB-MP460054LMV
CSB-EP460054LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri N-acetyldiaminopimelate deacetylase (LAR_0594) CSB-YP460055LMV
CSB-EP460055LMV
CSB-BP460055LMV
CSB-MP460055LMV
CSB-EP460055LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Probable GTP-binding protein EngB (engB) CSB-YP460056LMV
CSB-EP460056LMV
CSB-BP460056LMV
CSB-MP460056LMV
CSB-EP460056LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Protein grpE (grpE) CSB-YP460057LMV
CSB-EP460057LMV
CSB-BP460057LMV
CSB-MP460057LMV
CSB-EP460057LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Aspartate--tRNA ligase (aspS), partial CSB-YP460058LMV
CSB-EP460058LMV
CSB-BP460058LMV
CSB-MP460058LMV
CSB-EP460058LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP460059LMV
CSB-EP460059LMV
CSB-BP460059LMV
CSB-MP460059LMV
CSB-EP460059LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP460060LMV
CSB-EP460060LMV
CSB-BP460060LMV
CSB-MP460060LMV
CSB-EP460060LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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