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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum Proline--tRNA ligase (proS), partial CSB-YP460821DUF
CSB-EP460821DUF
CSB-BP460821DUF
CSB-MP460821DUF
CSB-EP460821DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S12 (rpsL) CSB-YP460822DUF
CSB-EP460822DUF
CSB-BP460822DUF
CSB-MP460822DUF
CSB-EP460822DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L16 (rplP) CSB-YP460823DUF
CSB-EP460823DUF
CSB-BP460823DUF
CSB-MP460823DUF
CSB-EP460823DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S8 (rpsH) CSB-YP460824DUF
CSB-EP460824DUF
CSB-BP460824DUF
CSB-MP460824DUF
CSB-EP460824DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L13 (rplM) CSB-YP460825DUF
CSB-EP460825DUF
CSB-BP460825DUF
CSB-MP460825DUF
CSB-EP460825DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP460826DUF
CSB-EP460826DUF
CSB-BP460826DUF
CSB-MP460826DUF
CSB-EP460826DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribonuclease PH (rph) CSB-YP460827DUF
CSB-EP460827DUF
CSB-BP460827DUF
CSB-MP460827DUF
CSB-EP460827DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable septum site-determining protein MinC (minC) CSB-YP460828DUF
CSB-EP460828DUF
CSB-BP460828DUF
CSB-MP460828DUF
CSB-EP460828DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphopentomutase (deoB) CSB-YP460829DUF
CSB-EP460829DUF
CSB-BP460829DUF
CSB-MP460829DUF
CSB-EP460829DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP460830DUF
CSB-EP460830DUF
CSB-BP460830DUF
CSB-MP460830DUF
CSB-EP460830DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribonuclease P protein component (rnpA) CSB-YP460831DUF
CSB-EP460831DUF
CSB-BP460831DUF
CSB-MP460831DUF
CSB-EP460831DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein hfq (hfq) CSB-YP460832DUF
CSB-EP460832DUF
CSB-BP460832DUF
CSB-MP460832DUF
CSB-EP460832DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal protein L11 methyltransferase (prmA) CSB-YP460833DUF
CSB-EP460833DUF
CSB-BP460833DUF
CSB-MP460833DUF
CSB-EP460833DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Potassium-transporting ATPase A chain (kdpA), partial CSB-YP460834DUF
CSB-EP460834DUF
CSB-BP460834DUF
CSB-MP460834DUF
CSB-EP460834DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glycerol kinase (glpK) CSB-YP460835DUF
CSB-EP460835DUF
CSB-BP460835DUF
CSB-MP460835DUF
CSB-EP460835DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP460836DUF
CSB-EP460836DUF
CSB-BP460836DUF
CSB-MP460836DUF
CSB-EP460836DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP460837DUF
CSB-EP460837DUF
CSB-BP460837DUF
CSB-MP460837DUF
CSB-EP460837DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S16 (rpsP) CSB-YP460838DUF
CSB-EP460838DUF
CSB-BP460838DUF
CSB-MP460838DUF
CSB-EP460838DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP460839DUF
CSB-EP460839DUF
CSB-BP460839DUF
CSB-MP460839DUF
CSB-EP460839DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP460840DUF
CSB-EP460840DUF
CSB-BP460840DUF
CSB-MP460840DUF
CSB-EP460840DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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