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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nostoc punctiforme Putative pterin-4-alpha-carbinolamine dehydratase (Npun_F4554) CSB-YP462222NHQ
CSB-EP462222NHQ
CSB-BP462222NHQ
CSB-MP462222NHQ
CSB-EP462222NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme FO synthase subunit 2 (cofH) CSB-YP462223NHQ
CSB-EP462223NHQ
CSB-BP462223NHQ
CSB-MP462223NHQ
CSB-EP462223NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Putative membrane protein insertion efficiency factor (Npun_R6368) CSB-YP462224NHQ
CSB-EP462224NHQ
CSB-BP462224NHQ
CSB-MP462224NHQ
CSB-EP462224NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme GTPase obg (obg) CSB-YP462225NHQ
CSB-EP462225NHQ
CSB-BP462225NHQ
CSB-MP462225NHQ
CSB-EP462225NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (Npun_F6457) CSB-YP462226NHQ
CSB-EP462226NHQ
CSB-BP462226NHQ
CSB-MP462226NHQ
CSB-EP462226NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme ATP synthase subunit c (atpE), partial CSB-YP462227NHQ1
CSB-EP462227NHQ1
CSB-BP462227NHQ1
CSB-MP462227NHQ1
CSB-EP462227NHQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme ATP synthase gamma chain (atpG) CSB-YP462228NHQ
CSB-EP462228NHQ
CSB-BP462228NHQ
CSB-MP462228NHQ
CSB-EP462228NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit L, partial CSB-YP462229NHQ1
CSB-EP462229NHQ1
CSB-BP462229NHQ1
CSB-MP462229NHQ1
CSB-EP462229NHQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP462230NHQ
CSB-EP462230NHQ
CSB-BP462230NHQ
CSB-MP462230NHQ
CSB-EP462230NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP462231NHQ
CSB-EP462231NHQ
CSB-BP462231NHQ
CSB-MP462231NHQ
CSB-EP462231NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Cell division protein SepF (sepF) CSB-YP462232NHQ
CSB-EP462232NHQ
CSB-BP462232NHQ
CSB-MP462232NHQ
CSB-EP462232NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem I assembly protein ycf3 (ycf3) CSB-YP462233NHQ
CSB-EP462233NHQ
CSB-BP462233NHQ
CSB-MP462233NHQ
CSB-EP462233NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Lysine--tRNA ligase (lysS), partial CSB-YP462234NHQ
CSB-EP462234NHQ
CSB-BP462234NHQ
CSB-MP462234NHQ
CSB-EP462234NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribonuclease Z (rnz) CSB-YP462235NHQ
CSB-EP462235NHQ
CSB-BP462235NHQ
CSB-MP462235NHQ
CSB-EP462235NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme DNA ligase (ligA), partial CSB-YP462236NHQ
CSB-EP462236NHQ
CSB-BP462236NHQ
CSB-MP462236NHQ
CSB-EP462236NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Dihydroxy-acid dehydratase (ilvD), partial CSB-YP462237NHQ
CSB-EP462237NHQ
CSB-BP462237NHQ
CSB-MP462237NHQ
CSB-EP462237NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem II reaction center protein M (psbM), partial CSB-YP462238NHQ
CSB-EP462238NHQ
CSB-BP462238NHQ
CSB-MP462238NHQ
CSB-EP462238NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem I reaction center subunit IX (psaJ), partial CSB-YP462239NHQ
CSB-EP462239NHQ
CSB-BP462239NHQ
CSB-MP462239NHQ
CSB-EP462239NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit O CSB-YP462240NHQ
CSB-EP462240NHQ
CSB-BP462240NHQ
CSB-MP462240NHQ
CSB-EP462240NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem I reaction center subunit IV (psaE) CSB-YP462241NHQ
CSB-EP462241NHQ
CSB-BP462241NHQ
CSB-MP462241NHQ
CSB-EP462241NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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