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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Exiguobacterium sibiricum Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP461821EPT
CSB-EP461821EPT
CSB-BP461821EPT
CSB-MP461821EPT
CSB-EP461821EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP461822EPT
CSB-EP461822EPT
CSB-BP461822EPT
CSB-MP461822EPT
CSB-EP461822EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP461823EPT
CSB-EP461823EPT
CSB-BP461823EPT
CSB-MP461823EPT
CSB-EP461823EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP461824EPT
CSB-EP461824EPT
CSB-BP461824EPT
CSB-MP461824EPT
CSB-EP461824EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Trigger factor (tig) CSB-YP461825EPT
CSB-EP461825EPT
CSB-BP461825EPT
CSB-MP461825EPT
CSB-EP461825EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP461826EPT
CSB-EP461826EPT
CSB-BP461826EPT
CSB-MP461826EPT
CSB-EP461826EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum tRNA (guanine-N (7)-)-methyltransferase CSB-YP461827EPT
CSB-EP461827EPT
CSB-BP461827EPT
CSB-MP461827EPT
CSB-EP461827EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S20 (rpsT) CSB-YP461828EPT
CSB-EP461828EPT
CSB-BP461828EPT
CSB-MP461828EPT
CSB-EP461828EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Probable cytosol aminopeptidase (pepA) CSB-YP461829EPT
CSB-EP461829EPT
CSB-BP461829EPT
CSB-MP461829EPT
CSB-EP461829EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum UPF0365 protein Exig_0818 (Exig_0818), partial CSB-YP461830EPT1
CSB-EP461830EPT1
CSB-BP461830EPT1
CSB-MP461830EPT1
CSB-EP461830EPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L33 2 (rpmG2) CSB-YP461831EPT
CSB-EP461831EPT
CSB-BP461831EPT
CSB-MP461831EPT
CSB-EP461831EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Peptide chain release factor 2 (prfB) CSB-YP461832EPT
CSB-EP461832EPT
CSB-BP461832EPT
CSB-MP461832EPT
CSB-EP461832EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP461833EPT
CSB-EP461833EPT
CSB-BP461833EPT
CSB-MP461833EPT
CSB-EP461833EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP461834EPT
CSB-EP461834EPT
CSB-BP461834EPT
CSB-MP461834EPT
CSB-EP461834EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ribonuclease Y (rny), partial CSB-YP461835EPT
CSB-EP461835EPT
CSB-BP461835EPT
CSB-MP461835EPT
CSB-EP461835EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria L-rhamnose mutarotase (rhaM) CSB-YP461836BXP
CSB-EP461836BXP
CSB-BP461836BXP
CSB-MP461836BXP
CSB-EP461836BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP461837BXP
CSB-EP461837BXP
CSB-BP461837BXP
CSB-MP461837BXP
CSB-EP461837BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP461838BXP
CSB-EP461838BXP
CSB-BP461838BXP
CSB-MP461838BXP
CSB-EP461838BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Flagellar L-ring protein (flgH) CSB-YP461839BXP
CSB-EP461839BXP
CSB-BP461839BXP
CSB-MP461839BXP
CSB-EP461839BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria S-adenosylmethionine synthase (metK) CSB-YP461840BXP
CSB-EP461840BXP
CSB-BP461840BXP
CSB-MP461840BXP
CSB-EP461840BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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