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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Methionine--tRNA ligase (metG), partial CSB-YP462622SYZ
CSB-EP462622SYZ
CSB-BP462622SYZ
CSB-MP462622SYZ
CSB-EP462622SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP462623SYZ
CSB-EP462623SYZ
CSB-BP462623SYZ
CSB-MP462623SYZ
CSB-EP462623SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP462624SYZ
CSB-EP462624SYZ
CSB-BP462624SYZ
CSB-MP462624SYZ
CSB-EP462624SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP462625SYZ
CSB-EP462625SYZ
CSB-BP462625SYZ
CSB-MP462625SYZ
CSB-EP462625SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP462626SYZ
CSB-EP462626SYZ
CSB-BP462626SYZ
CSB-MP462626SYZ
CSB-EP462626SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L10 (rplJ) CSB-YP462627SYZ
CSB-EP462627SYZ
CSB-BP462627SYZ
CSB-MP462627SYZ
CSB-EP462627SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Thiazole synthase (thiG) CSB-YP462628SYZ
CSB-EP462628SYZ
CSB-BP462628SYZ
CSB-MP462628SYZ
CSB-EP462628SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Homoserine O-succinyltransferase (metA) CSB-YP462629SYZ
CSB-EP462629SYZ
CSB-BP462629SYZ
CSB-MP462629SYZ
CSB-EP462629SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cobalamin synthase (cobS), partial CSB-YP462630SYZ1
CSB-EP462630SYZ1
CSB-BP462630SYZ1
CSB-MP462630SYZ1
CSB-EP462630SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative ion-transport protein yfeO (yfeO), partial CSB-YP462631SYZ
CSB-EP462631SYZ
CSB-BP462631SYZ
CSB-MP462631SYZ
CSB-EP462631SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Peptidase E (pepE) CSB-YP462632SYZ
CSB-EP462632SYZ
CSB-BP462632SYZ
CSB-MP462632SYZ
CSB-EP462632SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 D-cysteine desulfhydrase (dcyD) CSB-YP462633SYZ
CSB-EP462633SYZ
CSB-BP462633SYZ
CSB-MP462633SYZ
CSB-EP462633SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP462634SYZ
CSB-EP462634SYZ
CSB-BP462634SYZ
CSB-MP462634SYZ
CSB-EP462634SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP462635SYZ
CSB-EP462635SYZ
CSB-BP462635SYZ
CSB-MP462635SYZ
CSB-EP462635SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP462636SYZ
CSB-EP462636SYZ
CSB-BP462636SYZ
CSB-MP462636SYZ
CSB-EP462636SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Elongation factor P (efp) CSB-YP462637SYZ
CSB-EP462637SYZ
CSB-BP462637SYZ
CSB-MP462637SYZ
CSB-EP462637SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 30S ribosomal protein S6 (rpsF) CSB-YP462638SYZ
CSB-EP462638SYZ
CSB-BP462638SYZ
CSB-MP462638SYZ
CSB-EP462638SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 D-tagatose-1,6-bisphosphate aldolase subunit gatZ (gatZ) CSB-YP462639SYZ
CSB-EP462639SYZ
CSB-BP462639SYZ
CSB-MP462639SYZ
CSB-EP462639SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP462640SYZ
CSB-EP462640SYZ
CSB-BP462640SYZ
CSB-MP462640SYZ
CSB-EP462640SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 SsrA-binding protein (smpB) CSB-YP462641SYZ
CSB-EP462641SYZ
CSB-BP462641SYZ
CSB-MP462641SYZ
CSB-EP462641SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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