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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L34 (rpmH) CSB-YP460961WBO
CSB-EP460961WBO
CSB-BP460961WBO
CSB-MP460961WBO
CSB-EP460961WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L29 (rpmC) CSB-YP460962WBO
CSB-EP460962WBO
CSB-BP460962WBO
CSB-MP460962WBO
CSB-EP460962WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L18 (rplR) CSB-YP460963WBO
CSB-EP460963WBO
CSB-BP460963WBO
CSB-MP460963WBO
CSB-EP460963WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 30S ribosomal protein S13 (rpsM) CSB-YP460964WBO
CSB-EP460964WBO
CSB-BP460964WBO
CSB-MP460964WBO
CSB-EP460964WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Glutamate--tRNA ligase 1 (gltX1) CSB-YP460965WBO
CSB-EP460965WBO
CSB-BP460965WBO
CSB-MP460965WBO
CSB-EP460965WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP460966OEV
CSB-EP460966OEV
CSB-BP460966OEV
CSB-MP460966OEV
CSB-EP460966OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP460967OEV
CSB-EP460967OEV
CSB-BP460967OEV
CSB-MP460967OEV
CSB-EP460967OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi GTPase Der (der) CSB-YP460968OEV
CSB-EP460968OEV
CSB-BP460968OEV
CSB-MP460968OEV
CSB-EP460968OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Octanoyltransferase (lipB) CSB-YP460969OEV
CSB-EP460969OEV
CSB-BP460969OEV
CSB-MP460969OEV
CSB-EP460969OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Heme A synthase (ctaA), partial CSB-YP460970OEV
CSB-EP460970OEV
CSB-BP460970OEV
CSB-MP460970OEV
CSB-EP460970OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S6 (rpsF) CSB-YP460971OEV
CSB-EP460971OEV
CSB-BP460971OEV
CSB-MP460971OEV
CSB-EP460971OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP460972OEV
CSB-EP460972OEV
CSB-BP460972OEV
CSB-MP460972OEV
CSB-EP460972OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP460973OEV
CSB-EP460973OEV
CSB-BP460973OEV
CSB-MP460973OEV
CSB-EP460973OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S17 (rpsQ) CSB-YP460974OEV
CSB-EP460974OEV
CSB-BP460974OEV
CSB-MP460974OEV
CSB-EP460974OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L18 (rplR) CSB-YP460975OEV
CSB-EP460975OEV
CSB-BP460975OEV
CSB-MP460975OEV
CSB-EP460975OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S11 (rpsK) CSB-YP460976OEV
CSB-EP460976OEV
CSB-BP460976OEV
CSB-MP460976OEV
CSB-EP460976OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Transcription elongation factor GreA (greA) CSB-YP460977OEV
CSB-EP460977OEV
CSB-BP460977OEV
CSB-MP460977OEV
CSB-EP460977OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Probable transcriptional regulatory protein OTT_1378 (OTT_1378) CSB-YP460978OEV
CSB-EP460978OEV
CSB-BP460978OEV
CSB-MP460978OEV
CSB-EP460978OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S4 (rpsD) CSB-YP460979OEV
CSB-EP460979OEV
CSB-BP460979OEV
CSB-MP460979OEV
CSB-EP460979OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Deoxycytidine triphosphate deaminase (dcd) CSB-YP460980OEV
CSB-EP460980OEV
CSB-BP460980OEV
CSB-MP460980OEV
CSB-EP460980OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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